The Journal of Phytopharmacology, 2020;9(6):410-418 ; DOI:10.31254/phyto.2020.9605
Pharmacognostical, Phytochemical Studies and In vitro antidiabetic Evaluation of Seed Extracts of Casuarina equisetifolia Linn.
S Muthuraj1 , P Muthusamy1 , R Radha1 , K Ilango2
1. Department of Pharmacognosy, College of Pharmacy, Madras Medical College, Chennai – 600003, India
2. Division of Phytochemistry and Pharmacognosy, Interdisciplinary Institute of Indian System of Medicine (IIISM), SRM Institute Science and Technology (SRMIST), Kattankulathur – 603 203.Kancheepuram (Dt), Tamil Nadu, India
*Author to whom correspondence should be addressed.
Received: 13th September, 2020 / Accepted: 8th November, 2020
Casuarina equisetifolia is a fast growing, evergreen pine tree. It is a well-known xerophytic plant of the tropics and subtropics region. It is endemic to coastal area and its origin from Australia. The current study has been done to examine the Pharmacognocstical, Phytochemical analysis and In vivo antidiabetic activity of successive solvent seed extract of Casuarina equisetifolia. The plant materials are collected from Coimbatore district and are authenticated by Siddha institute in Chennai. The pharmacognostical parameters such as Macroscopy, microscopy, powdermicroscopy, physiocemical constants and to determine the inorganic elements present in the seed. The seeds can be well dried and make into a coarse powder. Then it is subjected to continuous hot percolation method by using soxhlet apparatus. In successive solvent seed extraction, the extracts can be obtained from different types of polarity solvents namely non polar (hexane), mid polar (ethyl acetate) and polar solvent (ethanol). Its secondary metabolites are evaluated by preliminary Phytochemical analysis. The quantification of phyto constituents such as quercetin, Kaempferol, stigmasterol, Rutin, Ellagic acid are carried out by HPTLC, LC-MS. The antidiabetic activity of seed extract is confirmed by alpha amylase inhibitor assay and glucose uptake assay by using 3T3 L1 cell line.
Glucose uptake assay, Casuarinas equisetifolia, 3T3 L1cell line, Alpha amylase.
HOW TO CITE THIS ARTICLE
Muthuraj S, Muthusamy P, Radha R, Ilango K. Pharmacognostical, Phytochemical Studies and Invitro antidiabetic Evaluation of Seed Extracts of Casuarina equisetifolia Linn.. J Phytopharmacol 2020; 9(6):410-418.