ISSN: 2320-480X
Manuscript Submission

The Journal of Phytopharmacology, 2018;7(3):298-304 ;   DOI:10.31254/phyto.2018.7312

Research Article

Screening for community-acquired strains of methicillinresistant Staphylococcus aureus susceptible to extracts of Centaurea nigrescens

Luke G. Huggins1 , Kathryn D. Robinson1 , Kyra P. Lasko1 , Lauren B. Clower1 , Avery J. Gookin1 , Dustin E. Segraves1 , James C. Gainer1 , Grant P. Basagic1 , Kelly R. Machuca1 , Jacobo Rendon1

1. Department of Biology and Environmental Science, West Virginia Wesleyan College, Buckhannon, WV, USA

*Author to whom correspondence should be addressed.

Received: 30th May, 2018 / Accepted: 22nd June, 2018

Abstract


The rates of infection by community-acquired multi-drug resistant Staphylococcus aureus have risen dramatically over fifteen years in the United States. Community-acquired multi-drug resistant Staphylococcus aureus is responsible for rapidly progressive diseases, including necrotizing pneumonia, severe sepsis, and necrotizing fasciitis. Consequently, novel antibacterial strategies are needed to combat the rising antibiotic resistance seen in community-acquired multi-drug resistant strains. We have screened the Nebraska Transposon Mutant Library for MRSA strains that are either susceptible or resistant to methanol extracts of Centaurea nigrescens leaves and flowers. 10 strains containing mutations affecting transporter proteins were identified as having either significant resistance or susceptibility to Centaurea extract. Insertions in two different drug efflux transporter families have been identified. The EmrB/QacA drug resistance transporter subfamily is a multi-drug efflux pump responsible for the export of toxic molecules from bacteria and yeast. The ABC transporters are involved in drug import and export. These results confirm the effectiveness of the screen as a means for identifying drug-resistance genes affected by the C. nigrescens methanolic extract and suggest a role for drug efflux proteins in the resistance of S. aureus community-acquired multi-drug resistant Staphylococcus aureus to antibacterial plant metabolites.

Keywords

Centaurea, Antibacterial, MRSA, Staphylococcus aureus, Screen.


HOW TO CITE THIS ARTICLE

Huggins LG , Robinson KD , Lasko KP , Clower LB , Gookin AJ , Segraves DE , Gainer JC , Basagic GP , Machuca KR, Rendon J. Screening for community -acquired strains of methicillin -resistant Staphylococcus aureus susceptible to extracts of Centaurea nigrescens . J Phytopharmacol 2018; 7(3):298 -304 .

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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY 4.0) license. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/).

Copyright

Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.

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