The Journal of Phytopharmacology, 2017;6(5):260-263 ;   DOI:10.31254/phyto.2017.6501

Research Article

In vitro immunitary impact and antioxidant activity of aqueous extracts of Maprounea africana Müll (Euphorbiaceae) and Mitragyna stipulosa O.Kze (Rubiaceae)

Morabandza Cyr Jonas¹ , Nkounkou Loupangou Celestine^2,3 , Ongoka Pascal Robin³ , Ouamba Jean Maurille⁴ , Abena Ange Antoine¹ , Etou Ossibi Arnaud Wilfrid¹

Morabandza Cyr Jonas,Nkounkou Loupangou Celestine,Ongoka Pascal Robin,Ouamba Jean Maurille,Abena Ange Antoine,Etou Ossibi Arnaud Wilfrid

1. Biochemical and Pharmacological Laboratory, Health Sciences Faculty, Marien NGOUABI University, P.O.Box 69, Brazzaville-CONGO
2. Unity of Vegetable chemical and life, Sciences and Technical Faculty, Marien NGOUABI University, P.O.Box69, Brazzaville-CONGO
3. Departments of Exact Sciences, E.N. S, MarienNGOUABI University, P.O.Box 69, Brazzaville-CONGO
4. Unity of Vegetable chemical and life, Sciences and Technical Faculty, Marien NGOUABI University, P.O.Box 69, Brazzaville-CONGO

*Author to whom correspondence should be addressed.

Received: 23rd September, 2017 / Accepted: 24th October, 2017

Abstract

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This study aims to investigate the in vitro immunitary impacts and antioxidant activity of aqueous extracts of Maprounea africana (Euphorbiaceae) leaves and Mitragyna stipulosa (Rubiaceae) stem barks. Impact on leukocyte cells (total lymphocyte, polynuclears, monocyte, NK, TCD8 and TCD4) was quantified by using flow cytometry and, antioxidant activity by quantification of hydrogen peroxide production after immunomarking of specific monoclonal antibodies. The results showed a significant descrease of total lymphocyte, polynuclear, NK, TCD8 and, a non-significant descrease of TCD4 and monocyte induces by aqueous extract of M. africana leaves. Whereas aqueous extract of the stem bark of M. stipulosa induces a significant increase of total lymphocyte, TCD4, NK, TCD8 and, a significant descrease of polynuclear and monocyte. The two extracts significantly reduce (p?0.001) the production of hydrogen peroxid by polynuclear, lymphocytes and monocytes. These results suggest an immunomodulatory and immunostimulant effect of M. africana and M. stipulosa respectively and, antioxidant activity. The present study established pharmacological evidence to support traditional uses of these two species and may open up the possibility of finding the new compounds against immunological desseases.

Keywords

Maprounea africana, Mitragyna stipulosa, Immunitary impact, Antioxidant.

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