The Journal of Phytopharmacology, 2014;3(5):316-320 ; DOI:10.31254/phyto.2014.3503
Evaluation of Salvia haematodes Linn for their antioxidant, anti-microbial, anti-lipoxygenase and anticholinesterase activity
Ali Rizwan1 , Rao Saeed1 , Taseer Ahmad2 , Aftab Ullah1 , Zaira Ather3 , Sohail Muhammad2 , Iradat Hussain2 , Usman Ali1
1. Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan
2. Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, Pakistan
3. Faculty of Pharmacy, University of Sargodha, Sargodha, Pakistan
*Author to whom correspondence should be addressed.
The indigenous medicinal plant Salvia haematodes Linn (SAL) was screened for its pharmacological activities. The crude extract fractions were prepared in methanol, n-hexane, di chloromethane (DCM) and n-butanol by cold maceration process. The antioxidant effect of the extract fractions was observed by 1-1- diphenyl 2-picryl hydrazine (DPPH) radical scavenging activity. The anti-microbial activity was observed by using disc diffusion method. Furthermore, anti-enzymatic activity was studied against LOX (anti-lipoxygenase), acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). The results exhibited that the n-hexane (0.5 mg/mL) and DCM (0.5 mg/mL) fractions of SAL showed 83.3 ± 0.8% and 91.56 ± 0.7 % inhibition of DPPH radical. Whereas, the n-butanol fraction of the crude extract did not show any effect. The methanolic extract (10 mg/disc) of SAL inhibited the growth of E. coli at 12±0.5 mm as compared to ciprofloxacin (17.3 ± 0.5 mm). Moreover, at concentration of 0.5 mg/mL, the n-hexane and DMC extracts of SAL inhibited 68.19±0.39 % and 75.8 ± 1.7% of LOX enzyme respectively, against standard Baicallin (93.7 ± 1.2 % ). The methanol fraction of SAL showed 77.95 ± 0.6 % of AChE inhibition, whereas, Eserine standard inhibited 91.2 ±1.1% of AChE. The methanolic fraction of SAL showed 51.9±0.4 % inhibition of BChE enzyme against standard Eserine (82.8±1.0%). However, the DCM and n-hexane fractions did not elucidate any activity. Therefore, it is concluded that SAL have different pharmacology activities that may be due to presence of certain pharmacologically active compound.
Salvia haematodes Linn, Anti-oxidant, Antimicrobial, LOX, AChE and BChE.
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