The Journal of Phytopharmacology, 2017;6(6):322-328 DOI:10.31254/phyto.2017.6603

Research Article

Hepatic tolerance of an ethyl acetate extract of Holarrhena floribunda (G. Don) Durand and Schinz leaves in Wistar Rats

Désiré D. Koudou¹ , Tra-Bi Irie Otis^2,3 , Kouadio Yao E.¹ , A.P. Yapo¹ , A.J. Djaman^2,4

Désiré D. Koudou,Tra-Bi Irie Otis,Kouadio Yao E.,A.P. Yapo,A.J. Djaman

1. Laboratory of Physiology, Pharmacology and Pharmacopoeia, Natural Sciences Research and Training Unity, Nangui Abrogoua University, 02 PO Box 801 Abidjan 02, (Côte d’Ivoire)
2. Biochemical Pharmacodynamic Laboratory, Biosciences Department, Felix Houphouët Boigny University, PO Box 582 Abidjan 22 (Côte d’Ivoire)
3. Biochimical Microbiological Laboratory, Department of Life and Earth Sciences, Higher Teacher training school, PO Box, 10 Abidjan 08- Côte d’Ivoire
4. Department of Clinical and fundamental Biochemistry of Pasteur Institute of Côte d’Ivoire, PO Box 490 Abidjan 01 (Côte d’Ivoire)

*Author to whom correspondence should be addressed.

Received: 7th October, 2017 / Accepted: 27th November, 2017

Abstract

Purpose: This study aims to evaluate the hepatic biochemical effects associated with the use of ethyl acetate extract of Holarrhena floribunda leaves as a traditional medicine. Methods: The rats were randomly divided into four groups of 10 (male control, female control, male test and female test) and combined with four groups of 5 (male control satellite, female control satellite, male test satellite and female test satellite) were force-fed for 90 days. The control and control satellite control rats received distilled water at a rate of 2 mL / 100g PC, and treated and treated satellite treated rats received the 1000 mg / kg PC dose of the acetate extract of ethyl leaves of Holarrhena floribunda. The blood collected each week during the first month, then at the end of each month as of the 2nd month allowed to assay liver serum markers such as ALT, ASAT, PAL, LDH, total bilirubin, direct bilirubin, albumin, glucose, HDL-cholesterol, LDL-cholesterol, total cholesterol and triglycerides. Results: The animals were well supported gavage of the extract since no sign of pathological manifestation was observed on them. The biochemical analysis indicated on the one hand a significant decrease in the level of total cholesterol, glucose (in both sexes), LDL cholesterol (in the female rats), and on the other hand an increase in the HDL cholesterol level (in both sexes), the rate of ASAT activities (in female rats) and PAL (in rats). On the other hand serum levels of total bilirubin, direct bilirubin, albumin, triglycerides, activities of ALT and LDH did not significantly vary. Microscopic observations of the liver tissue sections of the rats in the test lot did not show any lesions. Conclusion: Administration of the ethyl acetate extract of Holarrhena floribunda leaves to the rats for 90 days did not interfere with liver function or cause liver tissue damage.