ISSN: 2320-480X
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The Journal of Phytopharmacology, 2021;10(4):220-224 DOI:10.31254/phyto.2021.10401

Research Article

HPLC Estimation of Flavanoid (quercetin) of leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia

Praveen Garg1

1. Department of Science, Asst. Prof., VITS College, satna, (M.P.), India

*Author to whom correspondence should be addressed.

Received: 8th May, 2021 / Accepted: 9th June, 2021

Abstract


High performance liquid chromatography method is used for the estimation of flavanoid (quercetin) in leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia. The dried leaves and stem of both plants were extracted with methanol and ethanol solvent. The concentration of quercetin of methanolic and ethanolic leaves and stem extracts of plants performed by using acetonitrile and methanol 50:50 (V/V) solutions as mobile phase and used a flow rate of 1ml per min and absorbance at 256 nm. We conclude in this study that Ocimum sanctum and Tinospora cordifolia has significant amount of flavanoid (quercetin), which indicate the high antioxidant activity of both plants and used for further study.

Keywords


Flavanoid (quercetin), HPLC, Ocimum sanctum, Tinospora cordifolia.

HOW TO CITE THIS ARTICLE

Garg P. HPLC Estimation of Flavanoid (quercetin) of leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia. J Phytopharmacol 2021; 10(4):220-224. doi: 10.31254/phyto.2021.10401

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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY 4.0) license. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/).

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Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.

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