The Journal of Phytopharmacology 2024; 13(1):4-11 DOI:10.31254/phyto.2024.13102
Evaluation of secondary metabolites and chromatographic fingerprint profiling of stem bark of Holarrhena antidysenterica L.
Ganesh Pawar1 , Samiksha Parihar1,2 , Hari Om Saxena1
1. NWFP Discipline, SFM Division, ICFRE - Tropical Forest Research Institute, Jabalpur, Madhya Pradesh, India
2. School of Sciences, ITM University, Gwalior, Madhya Pradesh, India
*Author to whom correspondence should be addressed.
Received: 11th January, 2024 / Accepted: 27th February, 2024
Holarrhena antidysenterica is an important medicinal plant in the Ayurvedic system of medicine. The present study aimed to screen secondary metabolites qualitatively and quantitively and to develop chemical fingerprint profiles of stem bark of the target species. The phytochemical screening findings revealed that the methanolic extract of stem bark contained alkaloids, flavonoids, phenols, steroids, tannins, cardiac glycosides, and terpenoids. HPTLC fingerprint profiles were developed which were specific to the mobile phase Toluene: Ethyl acetate: Diethylamine (6.5:2.5:1 v/v/v) and Rf values. The fingerprints are a valuable tool for quality assurance since these can be used as biochemical markers to differentiate between authentic drugs and adulterants.
Phytochemical screening, Secondary metabolites, Conessine, Chemical fingerprints, H. antidysenterica
HOW TO CITE THIS ARTICLE
Pawar G, Parihar S, Saxena HO. Evaluation of secondary metabolites and chromatographic fingerprint profiling of stem bark of Holarrhena antidysenterica L. J Phytopharmacol 2024; 13(1):4-11. doi: 10.31254/phyto.2024.13102
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