The Journal of Phytopharmacology 2023; 12(3):143-151 ;   DOI:10.31254/phyto.2023.12302

Research Article

Antimicrobial activity, cytotoxicity, and qualitative phytochemical composition of aqueous and methanolic leaf extracts of Physalis peruviana L. (Solanaceae)

James M Mbaria¹ , Gervason A Moriasi^2,3 , Samuel M Njoroge⁴ , Gabriel O Aboge⁴

James M Mbaria,Gervason A Moriasi,Samuel M Njoroge,Gabriel O Aboge

1. Department of Public Health, Pharmacology, and Toxicology, College of Veterinary and Agricultural Sciences, University of Nairobi, P.O. Box 29053- 00625, Nairobi, Kenya
2. Department of Biochemistry, Microbiology, and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, P.O. Box 43844- 00100-G.P.O, Nairobi, Kenya.
3. Department of Medical Biochemistry, School of Medicine, College of Health Sciences, Mount Kenya University, P.O. 342-01000, Thika, Kenya
4. Department of Public Health, Pharmacology, and Toxicology, University of Nairobi, PO BOX 29053-00625, Nairobi, Kenya

*Author to whom correspondence should be addressed.

Received: 13th April, 2023 / Accepted: 28th May, 2023 / Published : 30th June, 2023

Abstract

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Background: The high morbidity and mortality associated with microbial infections and the ineffectiveness of conventional antibiotics due to inappropriate use and resistance warrant alternative stratagems. Aim: We investigated the antimicrobial, cytotoxicity, and qualitative phytochemical composition of the aqueous and methanolic leaf extracts of Physalis peruviana L. (Solanaceae) based on its ethnomedicinal information. Methods: The antimicrobial activity of the extracts was investigated using the disk diffusion technique. The brine shrimp lethality assay method was used to determine the cytotoxicity of the study extracts on brine shrimp nauplii. Qualitative phytochemistry of the study extracts was performed using standard procedures. Results: The two extracts did not possess antimicrobial activity against P. aeruginosa and were either inactive or slightly inactive against the other microbes at concentrations of ? 50 µg/ml. Notably, the aqueous extract exhibited high to very high activities against E. coli and S. aureus and very high to remarkable activities against B. cereus. The methanolic extract showed remarkable activity at concentrations of ?200 µg/ml against E. coli, moderate to very high against S. aureus, high to very high against B. cereus, and moderate to high against C. albicans. The aqueous extract's minimum inhibitory concentrations (MICs) were 50 ?g/ml (E. coli and B. cereus), 100 ?g/ml (S. aureus), and 200 ?g/ml (C. albicans). The minimum bactericidal concentration concentrations (MBCs) of the aqueous extract were 100 ?g/ml (E. coli and B. cereus), 200 ?g/ml (S. aureus), while the minimum fungicidal concentration (MFC) for C. albicans was 400 ?g/ml. The MICs of the methanolic extract were 25 ?g/ml for E. coli, S. aureus, and B. cereus and 100 ?g/ml for C. albicans, and the MBCs were 100 ?g/ml for E. coli, S. aureus, and B. cereus, and the MFC for C. albicans was 200 ?g/ml. The aqueous was non-toxic, while the methanolic extract was slightly toxic to brine shrimp nauplii, indicating their relative safety. Various phytochemicals were detected in the study extracts, depicting broad pharmacologic activity, including antimicrobial activity. Conclusion: The aqueous and methanolic leaf extracts of P. peruviana may serve as a source of efficacious and safe novel antimicrobial agents upon further evaluation.

Keywords

Disk diffusion technique, Brine Shrimp Lethality Assay, Minimum Inhibitory Concentration, Minimum Bacterial/Fungicidal Concentration (MBC/MFC), Phytochemicals

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