The Journal of Phytopharmacology 2026; 15(2):135-143 ; DOI:10.31254/phyto.2026.15203
HR-LCMS analysis of phytochemicals from Bignonia magnifica and evaluation of antioxidant activity using the FRAP method
Kyadagi Ravikumara1 , Taralabalu Matt Kaveri1 , S. Umesha1
1. Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru-570006, Karnataka, India
*Author to whom correspondence should be addressed.
Received: 6th January, 2026 / Accepted: 13th April, 2026 / Published : 17th April, 2026
Background: Bignonia magnifica, known as glow vine or purple funnel vine, is a stunning evergreen climber from the Bignoniaceae family, native to humid forests in Colombia, Ecuador, Panama, and Venezuela. It has an aesthetic value and potential medicinal properties. Objectives: The present study was carried out to evaluate phytochemical compositions and antioxidant activity of B. magnifica leaf extracts. Materials and Methods: Sequential leaf extraction was performed by Soxhlet apparatus using Chloroform, Ethyl acetate, Ethanol and Methanol solvents. The qualitative and quantitatively estimation of phytochemical constituents by using standard procedure. High Resolution- Liquid Chromatography Mass Spectroscopy analysis to identify bioactive compounds. The antioxidant activity was evaluated by ferric reducing antioxidant power assay. Results: Preliminary phytochemical screening of leaf extracts revealed the presence of carbohydrates, tannins, phenols, flavonoids, terpenoids, proteins, alkaloids, saponins, glycosides, and cardiac glycosides. The Quantitative analysis indicates that the ethyl acetate solution has a high phenolic content (49.50±0.07 mg/g) and tannin (506.00±0.041TAE) content. In contrast, the chloroform infusion shows increased flavonoid levels (4.00±0.001mg/gQE), terpenoids (1.42±0.002mg-TPE/g) and alkaloids (16.50±0.58%). The FRAP assay measures the antioxidant task and demonstrates that ethyl acetate (2241.07±0.01mg/gAAE) infusion has a greater diminishing effect than chloroform (1062.50±0.01 mg/gAAE) infusion. The HR-LCMS analysis identified bioactive compounds such as Genistein, Hydroquinidine, 7',8'-Dihydro-8' hydroxycitraniaxanthin, 14,19- Dihydroaspidospermatine, Pyrrhoxanthinol, Notoginsenoside R10. Conclusion: These findings indicate that the B. magnifica leaf contain a significant amount of bioactive substances and natural antioxidants, which supports the idea that the leaf could be used as a curative agent in the treatment of oxidative stress disorders and medicinal properties.
Bignonia magnifica, Phytochemical screening, Total phenolic content, Bioactive compounds, HR-LCMS analysis, Ferric reducing antioxidant power (FRAP).
HOW TO CITE THIS ARTICLE
Ravikumara K, Kaveri TM, Umesha S. HR-LCMS analysis of phytochemicals from B. magnifica and evaluation of antioxidant activity using the FRAP method. J Phytopharmacol 2026; 15(2):135-143. doi: 10.31254/phyto.2026.15203
Creative Commons (CC) License-
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY 4.0) license. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/).
Copyright
Copyright © 2026 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.