The Journal of Phytopharmacology, 2021;10(6):429-432 ;   DOI:10.31254/phyto.2021.10601

Research Article

Modulation of steroid hormone synthesis by alcoholic extract of Asparagus racemosus on MCF-7 cells

Dhanusha Gowda¹ , Sujith Samraj² , Nisha Aynikattil Ravindran³ , Raji Kanakkaparambil⁴ , Aathira Kadakkaparambil Krishnan⁵ , Haima Jumailathu Shahjahan¹ , Bibu John Kariyil²

Dhanusha Gowda,Sujith Samraj,Nisha Aynikattil Ravindran,Raji Kanakkaparambil,Aathira Kadakkaparambil Krishnan,Haima Jumailathu Shahjahan,Bibu John Kariyil

1. MVSc Scholar, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651, India
2. Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651, India
3. Assistant Professor and Head, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651, India
4. Associate Professor, Department of Veterinary Physiology College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651, India
5. Ph.D Scholar, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Kerala 680651, India

*Author to whom correspondence should be addressed.

Received: 21st October, 2021 / Accepted: 16th November, 2021

Abstract

---

The objective of the study was to elucidate the effect of alcoholic extract of A. racemosus on oestrogen and progesterone synthesis from MCF-7 cells. The root tubers of A. racemosus was collected dried, extracted in soxhlet, solvent evaporated and dried and was used for study. Qualitative phytochemical analysis of the extract was done. The IC50 was determined by doing MTT assay in MCF-7 cell lines. MCF-7 cells cultured in RPMI-1640 were exposed to IC50, half and double the doses of IC50 for 96 hours and the media collected every 48 hours to determine the concentration of oestrogen and progesterone using ELISA. The qualitative phytochemical analysis of the extract revealed the presence of steroids, alkaloids, diterpenes, triterpenes, tannins, glycosides and saponins The IC50 was found to be 267 µg/mL. The extract of A. racemosus caused an increase in the concentration of oestrogen and progesterone secreted by MCf-7 cells in a dose and time dependent fashion. There was an increase in the secretion of progesterone in a dose depenedent fashion compared to untreated cells, whereas the secretion decreased at 96 hours compared to 48 hours. From the study, it could be concluded that alcoholic extract of A. racemosus caused a positive modulation of steroid hormone synthesis.

Keywords

Asparagus racemosus, MCF-7, Oestrogen, Progesterone

---