The Journal of Phytopharmacology, 2017;6(2):140-148 ; DOI:10.31254/phyto.2017.6212
Hepatoprotective activity of methanolic extract of young shoots of Bambusa arundinaceae in thioacetamide induced liver injury in rats
Kajal N Chauhan1 , Bhavini Shah2 , Manish Nivsarkar3 , Anita A Mehta2
1. Department of Pharmacology, K. B. Raval College of Pharmacy, Gandhinagar, Gujarat-382423, India
2. Department of Pharmacology, L. M. College of Pharmacy, Ahmedabad, Gujarat-380009, India
3. Department of Pharmacology and toxicology, B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Ahmedabad, Gujarat380054, India
*Author to whom correspondence should be addressed.
Received: 4th April, 2017 / Accepted: 27th April, 2017
Background: Whole plant of Bambusa arundinaceae (Bamboo) belonging to family (Graminae) is used in number of disease including liver ailments in various parts of India. The present study is done to evaluate antioxidant and Hepatoprotective activity of methanolic extract of young shoots of Bambusa arundinaceae in Thioacetamide induced liver injury in rats. Material and method: Antioxidant activity of methanolic extract was evaluated by DPPH, Ferric reducing power assay and Lipid peroxidation. In vitro hepatoprotective activity of methanolic extracts (25, 50, 100, 200 and 400 µg/ml) against thioacetamide (100mM) induced cytotoxicity was assessed by monitoring cell viability in HepG2 and Hep3b tumor cell line and also in primary hepatocytes. In vivo hepatoprotective activity of methanolic extract (50, 100, 200 mg/kg p.o.) was observed againt thioacetamide (100 mg/kg s.c.) induced liver injury in rat by measuring biochemical parameters viz, Aspartate Transaminase, Alanine Transaminase, Alkaline Phosphatase, Total and Direct Bilirubin. The histopathological studies were also performed. Results: Methanolic extract of young shoots showed good antioxidant activity. Thioacetamide caused significant reduction in cell viability in in vitro studies. Treatment with methanolic extract caused significant increase in cell viability. In vivo studies showed that Thioacetamide induced elevation of biochemical parameters in rats were significantly (p
Hepatoprotective activity, Bambusa arundinaceae, Thioacetamide, HepG2, Hep3b, Primary hepatocytes.
HOW TO CITE THIS ARTICLE
Chauhan KN, Shah B, Nivsarkar M, Mehta AA. Hepatoprotective activity of methanolic extract of young shoots of Bambusa arundinaceae in thioacetamide induced liver injury in rats. J Phytopharmacol 2017;6(2):140-148.
Creative Commons (CC) License-
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY 4.0) license. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/).
Copyright
Copyright © 2017 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.