The Journal of Phytopharmacology, 2017;6(5):288-292 ; DOI:10.31254/phyto.2017.6506
Emodin, isolated and characterized from an endophytic fungus Polyporales sp., induces apoptotic cell death in human lung cancer cells through the loss of mitochondrial membrane potential
Refaz Ahmad Dar1 , Rabiya Majeed2 , Abid Ali sheikh1 , Shakeel-u-Rehman3 , Abid Hamid2 , Qazi Parvaiz Hassan2
1. Division of Biotechnology, Indian Institute of Integrative Medicine (CSIR), Sanatnagar, Srinagar, Jammu & Kashmir-190005, India
2. Division of Cancer Pharmacology, Indian Institute of Integrative Medicine (CSIR), Sanatnagar, Srinagar, Jammu & Kashmir-190005, India
3. Division of Natural Product Chemistry, Indian Institute of Integrative Medicine (CSIR), Sanatnagar, Srinagar, Jammu & Kashmir-190005, India
*Author to whom correspondence should be addressed.
Received: 30th September, 2017 / Accepted: 7th November, 2017
Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a Chinese herbal anthraquinone that exhibits numerous biological activities, such as antitumor, antibacterial, antiinflammatory, and immunosuppressive. From an endophytic fungus, a close relative of Polyporales sp., found in association with Rheum emodi, Wall. ex Meissn a compound (Rz) was isolated and characterizedby different spectroscopic techniques (1H-NMR, 13CNMR, 2D-NMR, and HRMS). The compound (Rz) displayed a range of cytotoxicities against different human cancer cell lines like THP-1(Leukemia), A549 (Lung), NCI-H322 (lung) and Colo-205(colon) at a concentration of 70 and 100 µM. The compound had strong anticancer activity by arresting the cell cycle at G1 and G2/M phase and loss of mitochondrial membrane potential in A-549 lung cancer cells in concentration dependent manner. The study suggests that emodin induced anticancer effects may have novel therapeutic applications for the treatment of lung cancer.
NMR, Cytotoxic, Rheum emodi, Cancer cell lines.
HOW TO CITE THIS ARTICLE
Dar AR, Majeed R, Sheikh AA, Shakeel-u-Rehman, Hamid A, Hassan QP. Emodin, isolated and characterized from an endophytic fungus Polyporales sp., induces apoptotic cell death in human lung cancer cells through the loss of mitochondrial membrane potential. J Phytopharmacol 2017;6(5):288-292.
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