The Journal of Phytopharmacology, 2014;3(5):343-347 ; DOI:10.31254/phyto.2014.3507
Antimicrobial activity of aqueous and methanol extract of naturally growing and cultivated Aloe turkanensis
Rukenya Zachary Muthii1 , Mbaria James Mucunu1 , Mbaabu Mathiu Pete2 , Kiama Stephen Gitahi3 , Okindo Ronald Onzago1
1. Department of Public Health, Pharmacology and Toxicology, University of Nairobi, P.O. Box 30197, Nairobi 00100, Kenya
2. Department of Veterinary Anatomy and Physiology, University of Nairobi, P.O. Box 30197, Nairobi 00100, Kenya
3. Department of Veterinary Anatomy and Physiology, University of Nairobi, P.O. Box 30197, Nairobi 00100, KenyaA
*Author to whom correspondence should be addressed.
The aim of the study was to determine the antibacterial properties of aqueous and methanol extracts of naturally occurring and cultivated Aloe turkanensis. The plant is widely used as a traditional herb by communities in Turkana County, Kenya. However its efficacy has not been established. Aqueous and methanol extract of a naturally occurring whole Aloe turkanensis and cultivated one was obtained. The extracts were tested for in-vitro activity against 4 standard bacterial cultures and a fungal clinical isolate. Benzyl penicillin, Gentamycin and Amphotericin B were used as positive controls. Efficacy data analysis showed that methanol extracts of naturally growing plant inhibited the growth of B. cereus (100 mg/ml), S. aureus (100 mg/ml), and P. aeroginosa (200 mg/ml) with mean diameters of inhibition zones for S. aureus and B. cereus being 18.5±0.7 mm and 16.5±0.7 mm, respectively. Aqueous extract of the plant inhibited the growth of B. cereus and S. aureus at a Minimum Inhibitory Concentration of 200 mg/ml and 50 mg/ml respectively with mean diameter of inhibition zones for S. aureus and B. cereus being 19.75±1.0 mm and 11.5±0.0 mm respectively. Methanol extracts of cultivated A. turkanensis inhibited the growth B. cereus (100 mg/ml), S. aureus (50 mg/ml), E. coli (400 mg/ml) and P. aeroginosa (200 mg/ml) with mean diameters of inhibition zones for S. aureus and B. cereus being 18.5±0.7 mm and 11.5±0.0 mm respectively. There was a significant difference in antibacterial activity between the two plant ecotypes (p
Aloe turkanensis, Antibacterial, Naturally occurring, Cultivated.
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