The Journal of Phytopharmacology, 2014;3(6):395-399 ; DOI:10.31254/phyto.2014.3604
Antioxidant and in vitro cytotoxic activity of extracts of aerial parts of Cocculus hirsutus (L) using cell line cultures (breast cell line)
Khushbu N. Thakkar1 , A K Prasad1 , Jyotiranjan Nayak1 , Satish V. Iyer2 , Shankul Kumar3
1. Department of Pharmacology, GHB Pharmacy college, Aniyad; Ta:Shehra, Panchmahal, Gujarat, India
2. General Manager-R&D, FOI Pharmaceuticals Pvt. Ltd. NGEF Layout, Banagalore, Karnatka, India
3. Department of Pharmacognosy, SGSPS Institute of Pharmacy, Kaulkhed, Akola, Maharastra, India
*Author to whom correspondence should be addressed.
The aim of the present study was to evaluate the in vitro cytotoxic activity of methanolic and aqueous extract of aerial parts of Cocculus hirsutus on MCF-7 breast cell lines. Qualitative phytochemical screening tests were performed to detect phytochemicals in the extracts. Antioxidant activity of the plant extracts were characterized by using DPPH free radical scavenging method. The cytotoxic activity of the extracts of Cocculus hirsutus on MCF-7 cells was investigated in vitro through MTT assay. The results showed Antioxidant activity using DPPH were found to be increased in a concentration dependent manner and decreased cell viability and cell growth inhibition in a dose dependent manner. The findings from this study indicated that methanolic and aqueous extracts of Cocculus hirsutus leaf possessed vast potential as a medicinal drug especially in breast cancer treatment.
Cocculus hirsutus, DPPH, MTT assay, Antioxidant, Cytotoxicity.
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