The Journal of Phytopharmacology 2023; 12(4):211-217 ; DOI:10.31254/phyto.2023.12401
Antiinflammatory, Antinociceptive and Antioxidant activities of aqueous and methanolic stem bark extracts of Fagaropsis hildebrandtii (Engl.) Milne-Redh. (Rutaceae)
Peter Maloba1 , Elias Mandela2 , Sammy Kimoloi3 , Jared Onyancha2
1. Department of medical Laboratory Sciences, Masinde Muliro University of Science and Technology, P.O Box 190-50100, Kakamega, Kenya
2. Department of Pharmacognosy, Mount Kenya University, P.O Box 342-01000 Thika, Kenya
3. Department of Medical Laboratory Sciences, Masinde Muliro University of Science and Technology, P.O Box 190-50100, Kakamega, Kenya
*Author to whom correspondence should be addressed.
Received: 6th June, 2023 / Accepted: 3rd August, 2023 / Published : 31st August, 2023
Background: Fagaropsis hildebrandtii has been widely used in African traditional medicine to treat various illnesses, particularly those associated with inflammation, pain and oxidative stress. However, experimental data on the anti-inflammatory, anti-nociceptive and antioxidant of the different parts of F. hildebrandtii is still limited. Objectives: The objectives of this study were to determine the phytochemical composition, anti-inflammatory, antinociceptive and antioxidant activities of stem bark extract of F. hildebrandtii from Makueni County, Kenya. Materials and Methods: The stem bark of F. hildebrandtii was extracted by maceration using water and methanol. Standard qualitative methods were then used to determine the presence or absence of various phytochemicals in the extracts. Total phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride calorimetric methods, respectively. Anti-inflammatory and anti-nociceptive activities of increasing water and methanol extracts doses (2-250mg/Kg) were tested using carrageenan-induced paw edema and acetic acid-induced writhing animal models, respectively in comparison with dexamethasone and aspirin as standard. Antioxidant activities of plant extracts were assayed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method, with ascorbic acid as the standard. Data analysis was conducted by two-way ANOVA with appropriate post hoc tests. Statistical significance level was set at p ? 0.05. Results: Phenols, flavonoids, alkaloids, saponins, tannins, terpenoids and coumarin were detected in the extracts. However, anthocyanins and anthraquinones were absent in both water and methanolic extracts. Notably, methanol extract had a high total phenolic level compared to water extract (34.590 ± 2.490 mgGAE/g vs. 7.893 ± 1.619 mgGAE/g; p0.05, two-way ANOVA). Conclusion: F. hildebrandtii has anti-inflammatory, antinociceptive and antioxidant activities. These findings validate the use of F. hildebrandtii crude extracts among Kenyan ethnic groups in management of diseases associated with inflammation, pain, and oxidative stress.
DPPH assay, Gallic acid, Paw edema, Phytochemisty, Traditional medicine
HOW TO CITE THIS ARTICLE
Maloba P, Mandela E, Kimoloi S, Onyancha J. Antiinflammatory, Antinociceptive and Antioxidant activities of aqueous and methanolic stem bark extracts of Fagaropsis hildebrandtii (Engl.) Milne-Redh. (Rutaceae). J Phytopharmacol 2023; 12(4):211-217. doi: 10.31254/phyto.2023.12401
Creative Commons (CC) License-
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY 4.0) license. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/).
Copyright
Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.