The Journal of Phytopharmacology, 2021;10(5):331-337 ; DOI:10.31254/phyto.2021.10509
Comparative study on the phytochemical composition and antibacterial activity of the essential oil of Diphasia klaineana stems at pre-flowering and fruit set
Benson Boua Boua1 , Yacouba Koné1 , Lanciné Traoré1,2 , N’Guessan Ambroise Malan3 , Janat Akhanovna MamyrbekovaBékro1 , Yves-Alain Békro1
1. Laboratoire de Chimie Bio-Organique et de Substances Naturelles, UFR-SFA, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire
2. Département de Sciences et Technologie, Université de Man, BP 20 Man, Côte d’Ivoire
3. Laboratoire de Chimie Organique Biologique, UFR-SSMT, Université Félix Houphouët-Boigny, BPV 34 Abidjan, Côte d’Ivoire
*Author to whom correspondence should be addressed.
Received: 22nd July, 2021 / Accepted: 9th August, 2021
The phytochemical composition and antibacterial activity of the essential oils of Diphasia klaineana at different developmental stages (pre-flowering and fruit set) were reported. The essential oils were extracted by continuous hydro distillation and their antibacterial activities were tested against Staphyloccocus aureus ATCC 25923. The yield of oil (w/w %) of stems in different stages was in the order: pre-flowering (0.21 %) > fruit set (0.07 %). GC and GC/MS were analyzed the essential oils composition. In total, 35 and 34 constituents were identified and quantified in the mentioned samples, respectively. Monoterpene hydrocarbons were the main group of compounds in the stems during preflowering (42.40 %) and fruit set (56.15 %). Major compounds at pre-flowering were ?-elemol, sabinene, guaiol and terpinen-4-ol. The antibacterial effect of essential oils was estimated by the disk diffusion method using Müller–Hinton agar and the measurement of diameters of inhibition zones. The bioassay results showed some variations between the two tested oils in their inhibitory activity against the tested bacteria at 10 µL. The essential oils from Diphasia klaineana stems at pre-flowering exhibited potent antibacterial activity against Staphyloccocus aureus ATCC 25923, with a minimum inhibitory concentration (MIC) value of 25 mg/mL, while the stems essential oil at fruit set had no activity.
Diphasia klaineana, Essential oils, Development stage, Phytochemical composition, Antibacterial activity, Staphylococcus aureus.
HOW TO CITE THIS ARTICLE
Boua BB, Koné Y, Traoré L, Malan NA, Mamyrbekova-Békro JA, Békro YA. Comparative study on the phytochemical composition and antibacterial activity of the essential oil of Diphasia klaineana stems at pre-flowering and fruit set. J Phytopharmacol 2021; 10(5):331-337. doi: 10.31254/phyto.2021.10509
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