The Journal of Phytopharmacology, 2020;9(4):258-264 DOI:10.31254/phyto.2020.9408

Research Article

Anti-ulcer activity of methanol extract of the leaves of Hannoa klaineana in rats

Ibrahim Abubakar¹ , Hassan Yankuzo Muhammad² , Yushau Baraya Shuaibu³ , Muazu Gusau Abubakar¹

Ibrahim Abubakar,Hassan Yankuzo Muhammad,Yushau Baraya Shuaibu,Muazu Gusau Abubakar

1. Department of Biochemistry, Faculty of Science, Usmanu Danfodiyo University Sokoto, Nigeria
2. Department of Medical Biochemistry, College of Health Sciences, Usmanu Danfodiyo University Sokoto, Nigeria
3. Department of Pathology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, Nigeria

*Author to whom correspondence should be addressed.

Received: 19th June, 2020 / Accepted: 15th July, 2020

Abstract

Ulcer is a common gastrointestinal disease affecting 5% of the world’s population. Hannoa klaineana is used locally in the management of many gastrointestinal disorders and fevers. The study was conducted to evaluate toxicity profile and anti-ulcer effect of methanol extract of the leaves of Hannoa klaineana (Simaroubaceae). Acute toxicity test was conducted according to OECD guideline 423 using the limit test dose (5000 mg/kg) for 14 days. Sub-chronic toxicity study was carryout according to OECD guideline 407 by daily oral administration of the extract (500 and 1000 mg/kg) for 28 days. Anti-ulcer effect of the extract (100, 200 and 400 mg/kg b.wt) was evaluated using ethanol and indomethacin induced gastric ulcer models. In acute toxicity test, 5000 mg/kg dose of the extract does not caused mortality nor any sign of toxicity observed in the rats, thus, the LD50 value of the extract was above 5000 mg/kg. While sub-chronic toxicity test, the extract demonstrated significant (p