The Journal of Phytopharmacology, 2020;9(5):323-328 DOI:10.31254/phyto.2020.9506

Research Article

Evaluation of antioxidant and antidepressant activity of hydro-alcoholic extract of Ximenia americana stem bark

Lucie A Togbossi¹ , Povi Lawson-Evi¹ , Abdoulatif Diallo² , Kwashie Eklu-Gadegbeku¹ , Kodjo Aklikokou³

Lucie A Togbossi,Povi Lawson-Evi,Abdoulatif Diallo,Kwashie Eklu-Gadegbeku,Kodjo Aklikokou

1. Laboratory of Physiology/Pharmacology University of Lome BP 1515 Togo
2. Healh Sciences Faculty University of Lome BP 1515 Togo
3. Laboratory of Physiology / Pharmacology University of Lome BP 1515 Togo

*Author to whom correspondence should be addressed.

Received: 24th July, 2020 / Accepted: 15th September, 2020

Abstract

The present study was undertaken to evaluate the antioxidant and antidepressant activity of hydroalcoholic extract of Ximenia americana in case of Sodium fluoride induced toxicity on ICR mices. Antioxidant activity of the plant was evaluated in vitro through 2,2’-Azobis (2-Amidino-propane) Dihydrochloride test, metal chelating assay and total antioxidant capacity and in vivo through evaluation of malondialdehyde content in brain and liver. Antidepressant activity was evaluated through tail suspension test. In vitro evaluation of antioxidant activity showed that hydro-alcoholic extract of stem bark of Ximenia americana possesses a total antioxidant capacity of 303.081 ± 4.946 mg equivalent ascorbic acid per gram. From 2, 2’-Azobis (2-Amidino-propane) Dihydrochloride and metal chelating tests, it emerged that the extract inhibits hemolysis and chelates metals concentration-dependent manner. Animals were randomly divided into four groups with five animals in each group. The control group received distilled water, group II received sodium fluoride at dose of 13.6 mg/kg body weight, group III and group VI: received respectively hydro-alcoholic extract of Ximenia americana at 250 mg/kg and 500 mg/kg with sodium fluoride during 7 days. Results showed sodium fluoride decrease animal’s body weight even at the presence of the extract, increased significantly animal’s inactivity time and malondialdehyde levels in liver and brain. Animal’s inactivity time and malondialdehyde levels were significantly improved at the presence of the hydro-alcoholic extract of Ximenia americana. Ximenia americana have potential to mitigate sodium fluoride toxicity