The Journal of Phytopharmacology, 2018;7(5):404-411 DOI:10.31254/phyto.2018.7501

Research Article

Kalanchoe pinnata aqueous extract safety and potential cardioprotective effects in isoprenaline treated rats

Orelien Sylvain Mtopi Bopda¹ , Nathalie Nadège Chendjou Koumtouzi¹ , Danielle Bilanda² , Désiré Paul Djomeni Dzeufiet² , Christian Tiambo Keambou¹ , Théophile Dimo²

Orelien Sylvain Mtopi Bopda,Nathalie Nadège Chendjou Koumtouzi,Danielle Bilanda,Désiré Paul Djomeni Dzeufiet,Christian Tiambo Keambou,Théophile Dimo

1. Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Buea, Cameroon
2. Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon

*Author to whom correspondence should be addressed.

Received: 7th August, 2018 / Accepted: 10th October, 2018

Abstract

Introduction: Kalanchoe pinnata (Crassulaceae) aqueous extract antihypertensive property has been proven. This study aimed to evaluate the safety and potential cardioprotective effects of the extract in isoproterenol treated rat. Methods: A total of 35 adult wistar rats were randomly and evenly distributed into groups 1-7 then treated for 28days. Control groups 1-3 received 10mL/kg water (per os, neutral), 150mg/kg isoproterenol (ISO, sc, 48 and 24hours prior to sacrifice, negative) and ISO plus 10mg/kg propranolol (per os, ten days prior to sacrifice, positive). Safety test groups (4-6) received K. pinnata extract (50, 100 and 200mg/kg per os). Cardioprotective test group 7 received K. pinnata (100mg/kg per os) and ISO. The sacrifice of rats was carried out on day 29 and blood collected for haematological and biochemical analyses. Liver, kidney and heart were collected weighed and preserved for histopathological analysis. Results: There was no observable sign of toxicity. No significant difference was observed between the relative weight of the heart from groups 1-7. The platelet value dropped from 794.7x10 /L (group 2) to 284x10 /L (group 7, p