The Journal of Phytopharmacology, 2014;3(1): 9-15 DOI:10.31254/phyto.2014.3102
Docking study of the Rohitukine for the prevention of peptic ulcer- A New Target
Sunil Kumar Mishra1 , Vaibhav Mishra2 , Gautam Palit2 , Anil Kumar Pandey3 , Akanksha Srivastava3 , CKM Tripathi3 , Snober S. Mir4 , Abbas Ali Mahdi5 , Santosh Kumar Agarwal5 , Vijai Lakshmi5
1. Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow226001, India
2. Pharmacology Division, CSIRCentral Drug Research Institute, Lucknow-226001, India
3. Fermentation Technology Division, CSIR-Central Drug Research Institute, Lucknow-226001, India
4. Department of Biotechnology Division, Integral University, Lucknow-226026, India
5. Department of Biochemistry, King George Medical University, Lucknow-226003, India
*Author to whom correspondence should be addressed.
Context: In our previous study we have suggested that Rohitukine attenuates gastric mucosal injury; however its exact mechanism has not yet been established. Objective: The aim of present study was to evaluate the gastro protective mechanism of Rohitukine. Materials and methods: Sprague dawley rats and guinea pigs weighing 180–200 g were used. Dudenol ulcer was observed through Histamine induced gastric ulcer in guinea pigs. Result and Discussion: The present study was considered to evaluate the anti-ulcerogenic properties of an alkaloid chromane, Rohitukine from Dysoxylum binectariferum. Moreover, we studied the role of Rohitukine on the cytosolic concentration of cAMP and Histamine level in parietal cell-enriched cell suspension in order to ascertain its mechanism of action. In addition, Rohitukine failed to show protection in histamine induced gastric ulcer. Conclusion: Our docking study revealed that Rohitukine moderately bind with CCK2 receptor with binding energy as compare with standard drug benzotript. Furthermore, anti-secretory mechanism of Rohitukine mediated apparently through bind with CCK2 and inhibited the acid secretion, is novel to our finding.
Peptic ulcer, CCK2 receptor, Docking study, Rohitukine, Dysoxylum binectariferrum.
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