The Journal of Phytopharmacology, 2021;10(3):151-155 DOI:10.31254/phyto.2021.10301

Research Article

Acute Toxicity Assessment of the methanolic leaf extract of Annona squamosa Bark in Male Albino Rats

Jamila Saleh¹ , Funsho Olowoniyi² , Ekpa Emmanuel¹ , Abdulrahman Abdullateef¹ , Muhibat Komolafe Bolanle¹ , Madinat Hassan¹

Jamila Saleh,Funsho Olowoniyi,Ekpa Emmanuel,Abdulrahman Abdullateef,Muhibat Komolafe Bolanle,Madinat Hassan

1. Department of Biology, Air Force Institute of Technology, Kaduna, Nigeria.
2. Department of Biochemistry, Kaduna State University, Kaduna, Nigeria.

*Author to whom correspondence should be addressed.

Received: 2nd January, 2021 / Accepted: 1st March, 2021

Abstract

Throughout the history of man, traditional and herbal method of treatment of diseases has been used without considering the dose effect. Therefore, this present study is an attempt on investigating the effect of different doses of Annona squamosa methanolic leaf extract on male wistar Rats especially the delicate organs. The work involves oral administration of different doses (10, 100, 1000, 1600, 2900, 5000 mg/kg body weight) of the extract to groups of rats according to Lorkes method. The animals were monitored for 30 days at every 24 hours interval in order to find the median lethal dose (LD50) of the extract. Internal organ-body weight ratios (OBR) of animals in the test groups were determined and compared with those of the control group. LD50 was found to be greater than 5000mg/kg body weight without any significant decrease (p>0.05) in body weight. Biochemical analysis of Aspartate amino transferase (AST), Alanine amino transferase (ALT), Albumin and globulin of animals administered with extract showed no significant difference compared to the control groups (p>0.05) but concentration of Alkaline Phosphatase (ALP) indicated obvious changes in the treated groups compared to the control groups (p