The Journal of Phytopharmacology, 2014;3(5):348-352 DOI:10.31254/phyto.2014.3508

Research Article

Analyzing the phytochemical composition of Justicia neesii (Ramam)

Nimmakayala Sridhar¹ , Suguna Lakshmi Duggirala¹ , Goverdhan Puchchakayala²

Nimmakayala Sridhar,Suguna Lakshmi Duggirala,Goverdhan Puchchakayala

1. Department of Pharmacology, Pydah College of Pharmacy, Kakinada, Andhra Pradesh, India
2. Department of Pharmacology, Vaagdevi College of Pharmacy, Hanamkonda, Thelangana, India

*Author to whom correspondence should be addressed.

Abstract

Phytochemical profile is an important aspect as it will give an over view of possible pharmacological properties of the plant. Justicia neesii is a plant belongs to Acanthaceae family, on which no significant phytochemical and pharmacological was done. The objective of the present study is to elucidate the phytochemical profile and analysis of antioxidant properties by TLC method. The phytochemical analysis was done for screening the maximum number of phytochemicals using standard methods. The TLC plates were developed with a solvent system containing methanol: chloroform: hexane at a ratio of 7:2:1. Ascorbic acid was used as positive control and a blank TLC plate was used as negative control in the experiment. The diluted DPPH in methanol was sprayed on the developed plates and observed under UV light. The preliminary phytochemical analysis shows the presence of flavonoids, glycosides, lactones, lignins, phenols, phytosterols, quinins, reducing sugars, saponins and terpinoids. The TLC analysis has shown the higher intensity of yellow color for the test spots which indicating the higher antioxidant potential of plant extract compared to standard ascorbic acid after treatment with DPPH solution. The plant is having good antioxidant potential. The plant was also composed of many significant phytochemicals.