The Journal of Phytopharmacology, 2015;4(2):80-86 DOI:10.31254/phyto.2015.4204
Protective effects of Centella asiatica against isoproterenol-induced myocardial infarction in rats: biochemical, mitochondrial and histological findings
Vinay Kumar1 , Vivek Babu2 , K. Nagarajan3 , Lalit Machawal4 , Umakant Bajaj5
1. Associate Professor, Head, Deptt. of Pharmacology, KIET School of Pharmacy, Ghaziabad, (UP)- 201206, India
2. Research Scholar, Deptt. of Pharmacology, KIET School of Pharmacy, Ghaziabad, (UP)- 201206, India
3. Professor & Head Deptt. of Pharm. Chemistry, KIET School of Pharmacy, Ghaziabad (UP) 201206, India
4. Department of Pharmacology, University Institute of Pharmaceutical Science, Panjab University, Chandigarh-160014, India
5. Principal, KIET School of Pharmacy, Ghaziabad, (UP)- 201206, India
*Author to whom correspondence should be addressed.
Myocardial infarction (MI) induced by isoproterenol (ISO) is a standardized model to study the beneficial effects of different drugs. The protective effect of Centella asiatica on ISO-induced cardiotoxicity and the antioxidant activity involved in the protection were investigated in rats. Wistar albino rats were used in the present study. Animals were randomly divided into six groups comprising of six animals in each group. MI was induced in rats with ISO (85 mg/kg, sc) twice at an interval of 24 hrs. ISO produced significant alterations in the creatine kinase, biochemical parameters and moderate necrosis in the heart. The effect of Centella asiatica oral treatment for 21 days at two doses (100 mg and 200 mg/kg, body weight) was evaluated against ISO -induced cardiac necrosis. Levels of marker enzymes (LDH and CK) were assessed in serum while antioxidant parameters viz., reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and malondialdehde (MDA) were assayed in heart homogenate. Significant myocardial necrosis, depletion of endogenous antioxidants and increase in serum levels of marker enzymes were observed in ISO-treated animals as compared with the normal control animals. Centella asiatica showed a significant cardioprotective activity by lowering the levels of serum marker enzymes and lipid peroxidation as well as elevated the levels of antioxidant enzymes. The findings of the present study demonstrated that the cardioprotective effects of Centella asiatica on ISO-induced oxidative damage may be due to an augmentation of the endogenous antioxidants and inhibition of lipid peroxidation of membrane.a
Antioxidant, Centella asiatica, Creatine kinase, Isoproterenol, Myocardial infarction.
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