The Journal of Phytopharmacology, 2015;4(5):248-252 DOI:10.31254/phyto.2015.4502

Research Article

Influence of microwave assisted extraction on antioxidant and antiplasmodial activities of Trichilia roka extracts

Olga Nana¹ , Jean Momeni² , Fabrice Fekam Boyom³ , Martin Benoît Ngassoum¹

Olga Nana,Jean Momeni,Fabrice Fekam Boyom,Martin Benoît Ngassoum

1. National Advanced School of AgroIndustrial Sciences, University of Ngaoundere, P.O. Box 455 Ngaoundere, Cameroon
2. Department of Chemistry, Faculty of Science, University of Ngaoundere, P.O. Box 454 Ngaoundere, Cameroon
3. Department of Biochemistry, Faculty of Science, University of Yaounde 1, P.O. Box 812 Yaounde, Cameroon

*Author to whom correspondence should be addressed.

Abstract

Antiplasmodial and antioxidant activities of extracts derived from Trichilia roka (Chiov) (Meliaceae) root bark were determined respectively in vitro and using two methods as well as 1,1-diphenyl-2-picrylhydrazyl radicalscavenging and ?-carotene-linoleate model systems. The microwave assisted extraction extract was more effective concern antioxydant activity than the antiplasmodial activity compare to conventional mechanical agitation extraction method. Extracts obtained by microwave assisted extraction showed a high total polyphenol content of 126766 ?g Equivalent of Gallic acid /g Dry Mater and a total flavonoid content of 789.22 ?g Equivalent of Quercetin/g dry mater but a low antiplasmodial activity with and inhibition concentration of 48.386 ?g /mL and 23.983 ?g/mL for mechanical agitation extract. The evaluation of the antioxidant properties of the two extracts showed that those obtained by microwave assisted extraction shown in the 1,1-diphenyl-2-picrylhydrazyl assay and in the ?-carotene bleaching test, the highest antioxidant activity respectively, with an antioxidant activity of 82.12% and with a radical-scavenging activity with inhibition percentage of 88.78%..