The Journal of Phytopharmacology, 2015;4(6):303-306 DOI:10.31254/phyto.2015.4606

Research Article

Antimicrobial screening of free and bound flavonoid from the bark of Terminalia arjuna

Priyanka Jaiswal¹ , Padma Kumar²

Priyanka Jaiswal,Padma Kumar

1. Research Scholar; Laboratory of plant tissue culture and secondary metabolites, Department of Botany, University of Rajasthan, Jaipur, Rajasthan-302004, India
2. Professor; Laboratory of plant tissue culture and secondary metabolites, Department of Botany, University of Rajasthan, Jaipur, Rajasthan-302004, India

*Author to whom correspondence should be addressed.

Abstract

Continuous use of antibiotics results in the increased number of antibiotic resistant strains of microorganisms. Thus, a diverse arsenal of new antimicrobial agents is urgently needed to combat the diminishing efficacy of existing antibiotics. Almost all parts of plants (roots, stem, leaves, flowers, fruits and seeds) have been reported to have one or the other important bioactive compound but very little has been reported from bark of the plant except for few. Present study is confined to explore bark of Terminelia arjuna for some bioactive compounds. T. arjuna bark was collected, air dried and soxhlet extracted by using standard method for flavonoid extraction. Bound and free flavonoid extracts were then screened for antimicrobial activity using Disc Diffusion Assay. MIC, MBC and TA of each extract was also calculated. Both bound and free flavonoid showed activity against all the selected pathogens but the maximum inhibition zone was observed against Agrobacterium tumifacians (IZ= 19mm, AI=1.461±0.010) & Bacillus subtilis (IZ= 16mm, AI= 1.230±0.098) by the bound and free flavonoid extract of the plant respectively. Results obtained advocates the use of bark of the selected plant for pharmaceutical purpose to combat upcoming resistant pathogens.