The Journal of Phytopharmacology, 2022;11(5):347-352 DOI:10.31254/phyto.2022.11506

Research Article

Isolation and toxicity evaluation of feruloyl ester and other triterpenoids from Synadenium glaucescens Pax

Frank Rwegoshora^1,2 , Faith Mabiki¹ , Francis Machumi³ , Musa Chacha⁴ , Bjarne Styrishave² , Claus Cornett²

Frank Rwegoshora,Faith Mabiki,Francis Machumi,Musa Chacha,Bjarne Styrishave,Claus Cornett

1. Department of Chemistry and Physics, College of Natural and Applied Sciences, Sokoine University of Agriculture, P. O. Box 3038 Morogoro Tanzania
2. Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2100 Copenhagen, Denmark
3. Department of Natural Products Development and Formulation, Institute of Traditional Medicine, Muhimbili University of Health and Allied Science, P.O. Box 65001, Dar es Salaam
4. School of Life Sciences and Bioengineering, The Nelson Mandela Institute of Science and Technology, P.O. Box 447, Arusha –Tanzania

*Author to whom correspondence should be addressed.

Received: 25th August, 2022 / Accepted: 2nd October, 2022 / Published : 31st October, 2022

Abstract

The use of plants as sources of drug agents is attributed by factors among which are the easyaccessibility to plants, less toxicity and little or no drug resistance. An improvement in both traditionalmedicine and drug discovery field necessitates investigation of pure compounds in any plant withmedicinal value. Synadenium glaucescens Pax of the family Euphorbiaceae is among the medicinal plantin Tanzania which are proven to contain bioactive compounds against microbial infections. Analysis ofethanolic and methanolic extracts of root and stem barks respectively aided to isolated six purecompounds (SG1- 6). These compounds were analyzed by both 1D, 2D NMR and GC-MS while theirspectral processing was achieved in the Bruker TopSpin 3.6.2. Among these compounds, one was aphenolic (hemicosanyl ferulate-SG1), three triterpenoids, (lupeol- SG2, epifriedelanol- SG4 and eupholSG5), one steroid (?-sitosterol- SG6) and a long chain alkene (1-nonacosene- SG2). Cytotoxicityevaluation by Brine shrimp lethality test (BLST) indicated the compounds under report were practicallynon-toxic.