The Journal of Phytopharmacology 2024; 13(2):154-159 DOI:10.31254/phyto.2024.13209

Research Article

Thermoxidized palm oil diet (TPO) induced protein derangements in rats is ameliorated by fresh palm oil (FPO) and Vitamin E

Imoh Emmanuel Ukoh¹ , Idongesit Bassey Umoh² , Enene Esu Ukpai³ , Samuel Kelechi Mobisson⁴ , Ikete Precious Whiskey¹ , Perowei Azosibe¹ , Azibaobom Karibi¹ , Ukrakpo Oghenemega Sophia¹ , Adienbo Emmemodi Nwadighi¹

Imoh Emmanuel Ukoh,Idongesit Bassey Umoh,Enene Esu Ukpai,Samuel Kelechi Mobisson,Ikete Precious Whiskey,Perowei Azosibe,Azibaobom Karibi,Ukrakpo Oghenemega Sophia,Adienbo Emmemodi Nwadighi

1. Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Federal University Otuoke, Otuoke, Bayelsa State, Nigeria
2. Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Cross River State, Nigeria
3. Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Cross River State University, Ogoja, Cross River State, Nigeria
4. Department of Human Physiology, Faculty of Basic Medical Sciences, Madonna University, Elele, Rivers State, Nigeria

*Author to whom correspondence should be addressed.

Received: 6th February, 2024 / Accepted: 24th April, 2024 / Published : 30th April, 2024

Abstract

Objective(s): The therapeutic efficacy of FPO and vitamin E were studied against TPO induced derangements in some proteins in rats. Materials and Methods: 60 male albino rats (140-160g) weight range, grouped (n=10) as: Control, TPO, FPO, Vitamin E, TPO treated with FPO (TPO+FPO) and Vitamin E (TPO+ Vitamin E). TPO and FPO (15g each) mixed with 85g rat feed was used to prepare FPO and TPO diet. Vitamin E was administered 200mg/kg daily by oral gavage. Animals were fed for 4 weeks, while group 5 and 6 were further treated with FPO and Vitamin E for another 4 weeks. At the end of the experiment blood were collected from sacrificed rats via cardiac puncture and protein indices were analyzed using serum. Results: Sero-protein analysis revealed a significant reduction in total protein, albumin, globulin and albumin-globulin ratio in TPO compared to control, FPO and Vitamin E, but was significantly increased in TPO+FPO and TPO+ Vitamin E compared to TPO. Total protein was significantly increased in TPO+FPO compared to TPO+ Vitamin E, while globulin was significantly increased in TPO+ Vitamin E compared to TPO+FPO. Total bilirubin and unconjugated bilirubin were significantly increased in TPO compared to control, FPO and Vitamin E, but was significantly reduced in TPO+FPO and TPO+ Vitamin E compared to TPO. Total bilirubin was significantly reduced in TPO+FPO compared to TPO+ Vitamin E. Conjugated bilirubin was significantly reduced in TPO compared to control, FPO and Vitamin E, but was significantly increased in TPO groups treated with FPO and Vitamin E in relation to TPO. Conclusion: FPO and vitamin E displayed therapeutic efficacy in ameliorating protein derangements instigated by TPO consumption.