The Journal of Phytopharmacology, 2021;10(5):385-392 ;   DOI:10.31254/phyto.2021.10518

Research Article

Studies of Warburgia ugandensis lyophilized crude extract with Trichoderma asperellum on tomato blight pathogens

Esther W Kamau¹ , Eric G Mworia¹ , Peter W Masinde¹ , John M Maingi²

Esther W Kamau,Eric G Mworia,Peter W Masinde,John M Maingi

1. Department of Agriculture, Meru University of Science and Technology, P.O. Box 972-60200, Meru, Kenya
2. Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University P.O. Box 43844- 00100 Nairobi, Kenya

*Author to whom correspondence should be addressed.

Received: 14th August, 2021 / Accepted: 29th September, 2021

Abstract

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Early and late blights in tomatoes are among the most destructive diseases. The causative agents are Alternaria solani and Phytophthora infestans respectively. Warburgia ugandensis extracts have been demonstrated to have wide prophylactic and curative use as biocontrol agents against fungal and bacterial pathogens in animals but not in plants. Trichoderma species have been used as biocontrol agents for many phytopathogens. Warburgia ugandensis stem bark samples were air-dried ground, weighed then soaked in distilled water. The soaked material was then filtered and filtrate lyophilized to obtain crude extract which was screened against A. solani and P. infestans both in vitro and in vivo. All assays were performed in triplicate. Preliminary phytochemical analysis of the extract and minimum inhibitory concentration (MIC) were determined. Data on the inhibition by the extract was analyzed using ANOVA and the differences between means separated by Tukey’s test (p

Keywords

Biocontrol, Consumers, Safety, Phytochemicals, Blight.

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