The Journal of Phytopharmacology, 2020;9(3):193-196 ; DOI:10.31254/phyto.2020.9307
Screening of some plant extract against Lasiodiplodia theobromae isolated from Musambi (Citrus sinensis)
1. Kamla Rai College, Gopalganj, Bihar, India
*Author to whom correspondence should be addressed.
Received: 14th April, 2020 / Accepted: 29th May, 2020
The fruits of Citrus sinensis L. contain a wide range of active ingredients like vitamin C, flavonoids, acids and volatile oils. They have bergapten which sensitizes the skin to sunlight. Such valuable fruits of Citrus sinensis L. were collected from different fruit markets of Gorakhpur district of Uttar Pradesh. 21 fungi were isolated from fruits of Citrus sinensis. Isolated fungi were also tested for their pathogenic nature and weight loss in fruits. Out of 21 isolated fungi, 12 were confirmed Koch’s postulate. Aspergillus flavus, A. niger and Lasiodiplodia theobromae caused highest loss of weight. Extract of different plant parts of different angiospermic families were tested for their antifungal activity against Lasiodiplodia theobromae by inverted plate method. Apium graveolens, Erythrina indica, Foeniculum vulgare, Pimpenella anisum, Pouzolgia indica and Spillanthes acmella were found to be 100% effective against test fungus. The Minimum Inhibitory Concentration (MIC) of extract was 5000 ppm.
Fruits, Koch’s postulate, Inverted plate method, antifungal activity, plant extracts, MIC.
HOW TO CITE THIS ARTICLE
Srivastava D. Screening of some plant extract against Lasiodiplodia theobromae isolated from Musambi (Citrus sinensis). J Phytopharmacol 2020; 9(3):193-196.
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