The Journal of Phytopharmacology, 2019;8(4):199-202 ; DOI:10.31254/phyto.2019.8409
Evaluation of the Anti-Diarrheal activity of the ethanolic seed extract of Annona muricata
Peace Doe1 , Andaratu Iddrisu1 , Peniel Lartey1 , Kuupolo Elijah1 , Samira Issaka1 , Darko Ansah Enock1
1. Department of Pharmacology and Toxicology, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema Ghana
*Author to whom correspondence should be addressed.
Received: 20th July, 2019 / Accepted: 15th August, 2019
Background: Diarrhea continues to be a public health problem in Ghana. The fruit and leaves of Annona muricata are used traditionally for the management of gastro-intestinal disorders. This study aims to investigate the ethanolic seed extract of Annona muricata for anti-diarrheal activity in rats. Method: Three different models were used to test for anti-diarrheal activity; gastrointestinal motility test, castor oilinduced diarrhea, and castor oil-induced gastro enteropooling. The observed effect of the extract at various doses (150mg/kg, 300mg/kg and 650mg/kg) was compared to both negative (distilled water 10ml/kg) and positive control (Loperamide hydrochloride 2mg/kg). Results: Annona muricata extract showed a dosedependent reduction in diarrhea compared to the standard antidiarrheal drug, Loperamide hydrochloride and distilled water. The percentage inhibition in gastrointestinal motility calculated in reference to the controls was 58.98%, 67.34% and 14.69% for the highest concentration of the extract, Loperamide hydrochloride, and distilled water respectively. The percentage inhibition in defecation compared to the controls was 35.59% and 38.98% for 650mg/kg Annona muricata, and Loperamide hydrochloride respectively. The extract was able to inhibit intestinal fluid accumulation which was greater than the effect observed by Loperamide hydrochloride. Conclusion: The results obtained confirm the antidiarrheal activity of the seed extract of Annona muricata and thus provides the scientific justification for the traditional use of this plant for the management of diarrhea.
Annona muricata, Ethanolic extract, Rats, Anti-diarrheal.
HOW TO CITE THIS ARTICLE
Doe P, Iddrisu A, Lartey P, Elijah K, Issaka S, Enock DA. Evaluation of the Anti-Diarrheal activity of the ethanolic seed extract of Annona muricata. J Phytopharmacol 2019; 8(4):199-202.
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