The Journal of Phytopharmacology, 2018;7(1):51-55 ;   DOI:10.31254/phyto.2018.7111

Research Article

Antioxidant and anticancer activities of an Aporphine alkaloid isolated from Alphonsea sclerocarpa

Suman Joshi DSD¹ , Chander Amgoth² , Surya Narayana S¹ , Padmavathi CH¹ , Madhavi J³ , Krishna Satya A¹

Suman Joshi DSD,Chander Amgoth,Surya Narayana S,Padmavathi CH,Madhavi J,Krishna Satya A

1. Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Preadesh-522510, India
2. School of Engineering Sciences and Technology, University of Hyderabad, Hyderabad, Telangana-500046, India
3. Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Gunturn Andhra Pradesh-522510, India

*Author to whom correspondence should be addressed.

Received: 8th December, 2017 / Accepted: 18th January, 2018

Abstract

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Herein, we report on the anticancer and antioxidant activity of an Aporphine alkaloid isolated from medicinal plant Alphonsea sclerocarpa. Conventional column chromatography and preparative HPLC methods were used to isolate and purify the alkaloid. Based on NMR, FTIR and MS/MS spectroscopic techniques the compound was identified as Crebanine. This is the first report on the presence of Crebanine from the leaves of Alphonsea sclerocarpa. Antioxidant activity was evaluated using DPPH and FRAP assay. The antioxidant activity was dose-dependent and activity increased with the increase in the concentration. The cytotoxicity assay was carried out using MTT assay. The results showed remarkable cytotoxic activity against K562 (CML-chronic myeloid Leukaemia blood cancer) cells with a % inhibition of 37.545 at 500µm concentration after an incubation of 24h.

Keywords

Alphonsea sclerocarpa, antioxidant activity, anticancer activity, MTT assay, DPPH assay, FRAP assay, Crebanine.

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