The Journal of Phytopharmacology, 2016;5(3):93-99 ;   DOI:10.31254/phyto.2016.5301

Research Article

Comparison of antiaspergillar activity of extracts of Tectona grandis Linn according to two antifungal susceptibility testing

Elisée Kporou Kouassi^1,2 , Ibourahema Coulibaly¹ , Gervais Melaine M'boh³ , Mathieu Kra Adou Koffi² , Joseph Djaman Allico^2,3 , Sitapha Ouattara² , Ilioara Oniga⁴

Elisée Kporou Kouassi,Ibourahema Coulibaly,Gervais Melaine M'boh,Mathieu Kra Adou Koffi,Joseph Djaman Allico,Sitapha Ouattara,Ilioara Oniga

1. Laboratory of Biochemistry and Microbiology, Bioactives Natural Substances, Jean Lorougnon Guédé University, Daloa, Côte d'Ivoire
2. Laboratory of Biochemical Phamacodynamy, Felix Houphouët Boigny University, Abidjan, Côte d'Ivoire
3. Department of Medical and fundamental Biochemistry, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire
4. Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania

*Author to whom correspondence should be addressed.

Abstract

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Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regularly evaluated in different Laboratories. And, the processes of evaluation of antifungal susceptibility are often different to one laboratory to another. This study was undertaken in the goal to compare parameters obtained (MIC and MFC) with two antifungal susceptibility testing. The first one is Agar slant double dilution tubes method (M1) and the second is disc diffusion method /broth dilution according document CLSI M38 -A2 supplemented to 2% glucose (M2). Ethanol (EtOH 70%), Methanol (MeOH) and n-Hexane (n-HexTG) extracts of leaves of Tectona grandis have been tested in comparison to Itraconazole against two clinicals strains of Aspergillus fumigatus 896/AB and Aspergillus flavus 1006/OM isolated to HIV patients. Results showed that on A. flavus, agar slant method (M1) gave MIC ranging between 200 µg/mL and 800 µg/mL while disc diffusion meth/broth dilution (M2) showed growth inhibition between 100 µg/mL and 400 µg/mL. On A. fumigatus, inhibition was observed between 200µg/mL and 400 µg/mL with M2 in contrary M1 gave MIC located between 400 µg/mL and 800 µg/mL. For each strain tested, according method M2, MIC and MFC are identical and with method M1, these parameters were different. Evaluation of Itraconazole on each strain gave with M2, MIC= 50 µg/mL while inhibition has been showed at 100 µg/mL and 200µg/mL with M1. Also considering extracts, hydroalcoholic extract of T. grandis (EtOHTG) showed antifungal susceptibility testing less 2 to 4 efficiency than Itraconazole. Disc diffusion method/broth dilution (M2) according CLSI M38-A2 to evaluate Tectona grandis antifungal susceptibility is the process of evaluation which allo wed having lo west antifungal parameters (MIC and MFC) on A. flavus and A. fumigatus.

Keywords

Antifungal susceptibility testing, Extract, Slant method.

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