The Journal of Phytopharmacology, 2013;2(6): 14-18 ; DOI:10.31254/phyto.2013.2603
Antifungal activity of Bacillus species in bio-control of different plant pathogens
N. Revathi1 , M. Kalaiselvi1 , D. Gomathi1 , G. Ravikumar2 , C. Uma3
1. Department of Biochemistry, Karpagam University, Coimbatore 641021, India
2. Department of Biochemistry, Karpagam University, Coimbatore 641021, Indiaax
3. Associate Professor, Hawasaa University, Ethiopia
*Author to whom correspondence should be addressed.
Background: Plant diseases are caused mainly by fungi, bacteria, viruses and nematodes. Biocontrol of plant disease involves the use of an organism or organisms to reduce disease which includes management of resident populations of organisms (the 'black box' approach) and introductions of specific organisms (the 'silver bullet' approach) to reduce disease. Aim: In the present study we focus on the inhibitory activity of different strains of Bacillus sp.101, Bacillus sp.102, Streptomyces sp.NCL Aspergillus sp. MY 2, Chaetomium sp. MY3, Volutella sp. against some plant pathogens such as B. poitrasii, S. rolfsii, A. niger, Fusarium in In vitro. Methods: Isolation of microbial species and inhibitor studies were carried out by using standard protocols. Results: Among the tested bacterial cultures, two Bacillus strains, 101 and 102 showed positive hyphal tip bursting test for all the four fungal species. Streptomyces isolate NCL1 exhibited HTB for B. poitrasii and S. rolfsii. The Actinomyces isolate NCL 2 and fungal isolates, Aspergillus MY2 and Chaetomium MY3 showed positive test against B. poitrasii only. Conclusion: The result of this study supports the use of bio-control agents, not only because they are environmentally friendly, but because they are also effective in controlling the plant pathogen B. poitrasii and S. rolfsii.
Hyphal tip bursting; Inhibitor; Plant pathogens; Bacillus species.