The Journal of Phytopharmacology, 2022;11(4):300-309 ; DOI:10.31254/phyto.2022.11414
de-O-methyllasiodiplodin from Ludwigia hyssop folia causes death of human liver cancer cells through the mitochondrial apoptotic, Akt/NF-?B and STAT3 pathway in vitro
Yu Peng1 , Yuruo Gong1 , Congwei Wang1 , Dujuan Shi1 , Jinyan Zhang1 , G Wei Kevin Zhang1 , Xinzhou Yang1 , Xiaojun Li1
1. School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China
*Author to whom correspondence should be addressed.
Received: 10th May, 2022 / Accepted: 23rd June, 2022
(+)-(R)-de-O-methyllasiodiplodin (DOML), isolated from the Chinese herbal medicine Ludwigia hyssopfolia, has great potential for development in pharmacological research on hepatocellular carcinoma(HCC). In our study, the CCK-8 assay, morphological observation, flow cytometry (also known asAnnexin V-FITC/PI double staining), as well as Western blotting were adopted to study the anti-livercancer activity and mechanisms of DOML on HepG2 and HuH-7 cells. The research exhibited thatDOML dose- and time-dependently reduced the cell viability of HCC cells. DOML treatment resulted inchanges in cell morphology, such as irregular edges, reduced volume, and decreased adhesion wereobserved under the microscope. Flow cytometry analysis indicated that apoptosis is the major form ofcell death. In addition, blocking autophagy and necroptosis pathways couldn’t alleviate DOML-inducedapoptosis. Protein expression levels of Bax, activated Caspase-3 and Caspase-9, and PARP wereincreased, while Bcl-2 protein levels were reduced by DOML treatment, which suggested that themitochondrial apoptotic pathway may be involved in DOML-induced cell death. Moreover, theexpression of NF-?B and the phosphorylation of Akt and STAT3 decreased with the increase of dosage,suggesting that the apoptotic mechanism might be related to the Akt/NF-?B and STAT3 signalingpathways. All these results indicate that DOML has the potential effects of anti-hepatoma.
Anti-Cancer; de-O-methyllasiodiplodin; Apoptosis; Mitochondrial Pathway; Akt/NF-?B; STAT3
HOW TO CITE THIS ARTICLE
Peng YU, Gong Y, Wang C, Shi D, Zhang J, Zhang WK, Yang X, Xiaojun LI. de-Omethyllasiodiplodin from Ludwigia hyssopifolia causes death of human liver cancercells through the mitochondrial apoptotic, Akt/NF-?B and STAT3 pathway in vitro. JPhytopharmacol 2022; 11(4):300-309. doi: 10.31254/phyto.2022.11414
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