The Journal of Phytopharmacology 2025; 14(4):215-222 ; DOI:10.31254/phyto.2025.14402
Evaluation of chronic toxicity study of Nathaichoori Chooranam- a Siddha polyherbal formulation
G. Dayanand Reddy1 , Sathiyarajeswaran P2 , R.Ganesan1 , K. Venkatraman1 , T.Varalakshmi1 , R. Rohinkumar1 , S. Sevanthi1 , Arshad Ali1 , A. Jaya Prasanna Kumar1
1. Siddha Central Research Institute, Central Council for Research in Siddha, Arumbakkam, Chennai- 600106, Tamil Nadu, India
2. Siddha Regional Research Institute, Central Council for Research in Siddha, Puducherry- 605013, Puducherry, India
*Author to whom correspondence should be addressed.
Received: 4th August, 2025 / Accepted: 28th August, 2025 / Published : 30th September, 2025
Background: Nathaichoori Chooranam (NC), a Siddha formulation, is traditionally used in South India for hyperlipidemia, obesity and digestive disorders. NC, which is rich in phytochemicals such as alkaloids and flavonoids, shows promise in managing metabolic conditions. However, chronic toxicity studies are essential to confirm long-term safety, as long-term use of herbal medicines may lead to adverse effects. Objective: This study evaluates the safety profile of NC in a chronic toxicity model to support its therapeutic application. Materials and Methods: A 90-day chronic toxicity study was conducted using Wistar rats following OECD Guideline 452. Animals were randomized into 6 groups (n=10/sex/group): control (0.5% CMC vehicle) and dose groups (low, intermediate, high; 300, 600, 1000 mg/kg body weight, respectively) of Nathaichoori powder administered orally daily. The parameters assessed included body weight, food intake, hematological and biochemical markers (liver, kidney function), organ weights and histopathology of vital organs (liver, kidney, heart, spleen). Clinical signs and mortality were monitored daily. Results: No mortality or significant clinical abnormalities were observed in the groups. Body weight and food intake were comparable to controls. Hematological parameters (RBC, WBC, hemoglobin) and biochemical markers (ALT, creatinine, urea) did not show dose-related changes. Organ weights were unchanged, and histopathological analysis did not reveal treatment-related lesions in the liver, kidneys or other organs, indicating no chronic toxicity at the doses tested. Conclusion: Nathaichoori Chooranam did not exhibit chronic toxicity in rats at doses up to 1000 mg/kg for 90 days, indicating its safety for long-term use. These findings support the potential of NC as a safe herbal remedy, but human studies are needed to confirm safety and establish therapeutic doses.
Biochemical paramters, Chronic toxicity study, Hematological parameters, Nathaichoori Chooranam
HOW TO CITE THIS ARTICLE
Reddy GD, Sathiyarajeswaran P, Gaanesan R, Venkatraman K, Varalakshmi T, Varalakshmi T, et al. Evaluation of chronic toxicity study of Nathaichoori Chooranam- a Siddha polyherbal formulation. J Phytopharmacol 2025; 14(4):215-222. doi: 10.31254/phyto.2025.14402
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