The Journal of Phytopharmacology 2025; 14(5):346-351 ; DOI:10.31254/phyto.2025.14507
Impact of fermented polyherbal supplementation on hematobiochemical profile and growth parameters in broiler chickens
Ankit R. Patel1 , Jatin H. Patel2 , Falguni D. Modi2 , Dharmesh R. Patel3 , Ankita D. Verma4
1. Department of Veterinary Pharmacology & Toxicology, College of Veterinary Science & and Animal Husbandry, Kamdhenu University, Navsari- 396450, Gujarat, India
2. Assistant Professor, Department of Veterinary Pharmacology & Toxicology, College of Veterinary Science & and Animal Husbandry, Kamdhenu University, Navsari- 396450, Gujarat, India
3. Department of Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari- 396450, Gujarat, India
4. Department of Animal Genetics & Breeding, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Navsari- 396450, Gujarat, India
*Author to whom correspondence should be addressed.
Received: 5th September, 2025 / Accepted: 9th October, 2025 / Published : 30th November, 2025
Background: Escherichia coli (E. coli) infection leads to substantial economic losses in the global poultry industry and the growing issue of antibiotic resistance poses a serious threat to human health. The emergence of antimicrobial resistance due to excessive antibiotic use in poultry production has intensified the search for safer, natural alternatives to control bacterial infections. Objective: This study was conducted to evaluate the hematobiochemical safety profile of a fermented polyherbal formulation in broiler chickens. Materials and Methods: A total of 60 one day old Vencobb 400 broiler chicks were randomly divided into two groups, each comprising 30 birds. Group 1 served as the environmental control and was fed a basal diet without any additional treatment. Group 2 served as test group and received fermented polyherbal extract in drinking water @100 ml/litre. The study was carried out over a period of 42 days. Results: Fermented polyherbal extract demonstrated notable in vitro efficacy against E. coli, with an MIC of 12.5%. Statistical comparisons revealed no significant differences (p > 0.05) between G2 and G1 across all measured parameters: Haemoglobin (g/dl), Total Erythrocyte Count (TEC ×10?), Total Leucocyte Count (TLC ×10³), differential white blood cell counts (Heterophils %, Lymphocytes %, Monocytes %, Eosinophils %, Basophils %) and serum biochemical parameters: aspartate transaminase (U/L), alanine transaminase (U/L), total protein (g/dL), uric acid (mg/dL) and albumin (g/dL). All values remained consistent with the control group, suggesting a good safety margin from a hematobiochemical perspective. Conclusion: Findings from this 42-day study indicated that the fermented polyherbal extract is not associated with any observable hepatotoxic or nephrotoxic effects in boiler chickens. Based on the comprehensive analysis of key haematobiochemical parameters, the administration of the fermented polyherbal extract to the test group (G2) for 42 days demonstrated a favorable safety profile relative to the environmental control group (G1).
Boiler chickens, Fermented polyherbal extract, Hematobiochemical, Escherichia coli
HOW TO CITE THIS ARTICLE
Patel AR, Patel JH, Modi FD, Patel DR, Verma AD. Impact of fermented polyherbal supplementation on hematobiochemical profile and growth parameters in broiler chickens. J Phytopharmacol 2025; 14(5):346-351. doi: 10.31254/phyto.2025.14507
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