The Journal of Phytopharmacology, 2018;7(2):175-179 DOI:10.31254/phyto.2018.7212

Research Article

Antiproliferative effect of aqueous bark extract of Oroxylum indicum L. on Vigna radiata L. (Green gram) seedlings

Luk Bahadur Chetry¹ , Manuj K Bharali¹

Luk Bahadur Chetry,Manuj K Bharali

1. Cell and Molecular Biology Section, Department of Zoology, Rajiv Gandhi University, Rono Hills, Doimukh791112, Arunachal Pradesh, India

*Author to whom correspondence should be addressed.

Received: 13th February, 2018 / Accepted: 13th March, 2018

Abstract

The present investigation was carried out to evaluate the antiproliferative activity of aqueous bark extract of Oroxylum indicum L. on Vigna radiata L. (Green gram) seedlings. For different concentrations of plant extract (250, 500, 1000 and 2500 µg/mL) were treated in a 24 well microplate containing the Vigna radiata L. seeds (n=30) for 24, 48 and 72 hours. Colchicine (50 µg/mL) was used as standard drug. Seed germination and seedlings growth were measured. Mitotic index was calculated for the proliferation of cells. The treatment with plant extracts significantly inhibits the germination of seeds, roots and shoots growth, and reduced the mitotic index in meristem ells of Vigna radiata L. at dose and time dependent manner. Qualitative phytochemical screenings of the aqueous stem bark extract of Oroxylum indicum L. revealed the presence of alkaloids, carbohydrates, glycosides, saponins, phytosterols, phenolic compounds and flavonoids. Saponins were found to be in high concentration in the extract.