The Journal of Phytopharmacology, 2018;7(3):305-311 DOI:10.31254/phyto.2018.7313

Research Article

RP-HPLC based evidences of rich sources of Phenolics and water-soluble vitamins in an annual sedge Cyperus compressus

Sudeshna Datta¹ , Tapan Seal² , BK Sinha³ , Soumen Bhattacharjee⁴

Sudeshna Datta,Tapan Seal,BK Sinha,Soumen Bhattacharjee

1. Industrial Section, Indian Museum, Botanical Survey of India, Kolkata, West Bengal, India
2. Plant Chemistry Department, Botanical Survey of India, A. J. C. Bose Indian Botanic Garden, Shibpur, Howrah, West Bengal, Indai
3. Botanical Survey of India, CGO Complex, DF Block, Sector-I, Salt Lake City, Kolkata, West Bengal, India
4. Plant Physiology & Biochemistry Research Laboratory, UGC Centre for Advanced Study, Department of Botany, The University of Burdwan, West Bengal, India

*Author to whom correspondence should be addressed.

Received: 28th March, 2018 / Accepted: 17th June, 2018

Abstract

The present study makes an effort to evaluate the content of water-soluble vitamins like ascorbic acid (C), thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6) and folic acid (B9) by HPLC of Cyperus compressus. The in vitro antioxidant activity was carried out in different solvent system of varying polarity. The 70% ethanol appeared to be the most potent solvent for extraction of antioxidant compounds. The total phenolic and flavonoid content were found 72.544 ± 0.144 mg/g dm and 12.930 ± 0.144 mg/g dm respectively in the plant. Simultaneous HPLC fingerprinting of some standard phenolic acids and flavonoids in the 70 % aqueous ethanol extract was also carried out. B vitamins ranged between 0.028 to 0.431 mg/100g dm. The results confirm that this annual sedge is an excellent source of antioxidant- based phytonutrients, which could be used for the good of human being at the same time it can be used as a vitamin supplement to our diet. The study also provides the scientific basis of traditional significance of Cyperus compressus.