The Journal of Phytopharmacology, 2022;11(4):286-288 DOI:10.31254/phyto.2022.11411
Improved method of DNA extraction from leaf and rhizome samples of black turmeric (Curcuma caesia) for molecular analysis
Vinod Kumar Sahu1 , Keerthi Tantawi1 , Swapnil Sapre1 , Nishi Mishra1 , Prakash N Tiwari1 , Prashant Gigaulia1 , Sushma Nema1 , Sharad Tiwari1
1. Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, 482004, India
*Author to whom correspondence should be addressed.
Received: 26th June, 2022 / Accepted: 3rd August, 2022
Curcuma caesia belongs to the genus Curcuma and the family of Zingiberaceae, which is a veryimportant but unexplored medicinal plant. It is locally known as black turmeric or kali halide and ismainly used by the local tribal community as traditional medicine for the health sector. The rhizome ofblack turmeric has wide applications in the economic pharma sector due to essential active ingredients.This research aims to standardize a rapid, simple and efficient protocol for DNA extraction in Curcumacaesia which can be used for another genus of Zingiberaceae to obtain DNA from leaf samples. Changesin the concentration of components of DNA extraction buffer have improved the quantity of DNA fromleaf samples compared to rhizomes. Extracted DNA samples also proved more efficient in PCRamplification of DNA barcode primers. The protocol developed in the present study is more efficient forleaf samples of Curcuma caesia compared to rhizome samples.
Black Turmeric, Curcuma caesia, DNA Isolation, Leaf, Rhizome
HOW TO CITE THIS ARTICLE
Sahu VK, Tantawi K, Sapre S, Mishra N, Tiwari PN, Gigaulia P, et al. Improvedmethod of DNA extraction from leaf and rhizome samples of black turmeric(Curcuma caesia) for molecular analysis. J Phytopharmacol 2022; 11(4):286-288.doi: 10.31254/phyto.2022.11411
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