The Journal of Phytopharmacology, 2022;11(5):335-340 DOI:10.31254/phyto.2022.11504
Activity of Sterols Isolated from the Leaves of Commiphora swynnertonii (Burtt) Against Some Nosocomial Infectious Bacteria
David Credo1 , Faith P. Mabiki1 , Francis Machumi2 , Musa Chacha3 , Claus Cornett4
1. Department of Chemistry and Physics, College of Natural and Applied ScienceSokoine University of Agriculture, P.O. BOX 3038 Morogoro, Tanzania
2. Department of Natural Products Development and Formulation, Institute of Traditional Medicine-Muhimbili University of Health and Allied Science, P.O. BOX 65001 Dar es Salaam, Tanzania
3. Department of Sustainable Agriculture and Biodiversity Ecosystem Management, School of Life Sciences and Bioengineering, Nelson Mandela African Institute of Science and Technology, P.O. BOX 447 Arusha, Tanzania
4. Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitates parken 2, 2100 Kobenhavn, Denmark
*Author to whom correspondence should be addressed.
Received: 12th August, 2022 / Accepted: 4th September, 2022 / Published : 31st October, 2022
Commiphora swynnertonii (Burtt) is traditionally used for medicinal purposes both in humans and animals in Tanzania. Previously, its leaves extract has been demonstrated antibacterial activity. However, there is limited information on identification of pure compounds responsible for its observed antibacterial activity. Therefore, this study was conducted to isolate and evaluate antibacterial activity of pure compounds isolated from its leaves extract. Colum chromatographic separation of its dichloromethane leaves extract afforded the isolation of two sterols namely, Cholest-7-en-3?-ol (1) and ?-sitosterol (2). Their structures were deduced by using NMR data experimentally obtained and comparison with spectral data available in literatures. The sterols were evaluated for activity against four nosocomial infectious bacteria namely; Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa to determine Minimum Inhibitory Concentrations (MIC) by broth microdilution method. Cholest-7-en-3?-ol (1) demonstrated strong activity against S. aureus (MIC= 0.5 mg/ml) and weak activities (MIC >2mg/ml) against other bacteria. ?-sitosterol (2) exhibited weak activities (MIC >2mg/ml) against all tested bacteria. Therefore, presence of antibacterial active compound(s) such as Cholest-7-en-3?-ol (1) among others in the leaves of C. swynnertonii can justify its earlier reported antibacterial activity and further confirms the claimed traditional uses of this plant in the treatment of bacterial infections.
?-sitosterol, Burseraceae, Cholest-7-en-3?-ol, Commiphora swynnertonii, Nosocomial Infectious Bacteria, Sterols
HOW TO CITE THIS ARTICLE
Credo D, Mabiki FP, Machumi F, Chacha M, Cornett C. Activity of Sterols Isolatedfrom the Leaves of Commiphora swynnertonii (Burtt) Against Some NosocomialInfectious Bacteria. J Phytopharmacol 2022; 11(5):335-342. doi:10.31254/phyto.2022.11504
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