The Journal of Phytopharmacology 2024; 13(1):20-27 DOI:10.31254/phyto.2024.13104

Research Article

Antimicrobial activity of tea processing effluents collected from various Kenyan factories

Thaddeus Mangenya^1,2 , Daniel Kariuki¹ , Johnson Kinyua¹ , Martin Obanda¹ , Simon Ochanda³ , Gervason Moriasi^4,5

Thaddeus Mangenya,Daniel Kariuki,Johnson Kinyua,Martin Obanda,Simon Ochanda,Gervason Moriasi

1. Department of Biochemistry, School of Biomedical Sciences, Jomo Kenyatta University of Agriculture and technology, Nairobi, Kenya
2. Department of Medical Biochemistry, School of Medicine, Kisii University, Kisii, Kenya
3. Kenya Agricultural and Livestock Research Organization- Tea Research Institute, (KALRO- TRI), Kericho, Kenya
4. Department of Biochemistry, Microbiology and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya
5. Department of Medical Biochemistry, School of Medicine, Mount Kenya University, Thika, Kenya

*Author to whom correspondence should be addressed.

Received: 16th December, 2013 / Accepted: 27th February, 2024

Abstract

The escalating global challenge of antibiotic resistance demands exploration into alternative sources for antimicrobials. This study investigated the often-overlooked tea waste samples generated during tea processing from Kenyan processing factories to uncover novel resources containing potent and effective antimicrobial compounds. In this study, we collected tea waste samples from various tea processing factories in Kenya and assessed their antimicrobial activity against various microorganisms using the disk diffusion assay. To quantify the efficacy of each sample, we determined growth inhibition zones and minimum inhibitory and bactericidal concentrations (MICs and MBCs). The study unveiled diverse levels of antimicrobial activity in tea waste samples against specific microorganisms. Notably, the fluff sample from Gitambo factory demonstrated potent antibacterial effects against Clostridium disporicum. Various samples exhibited a moderate response to both Streptococcus pyogenes and Escherichia coli, yet they showed minimal to no activity, where applicable, towards Staphylococcus aureus and Candida albicans. This study showed that some tea effluents, which are often discarded during processing, show antimicrobial potential, as they demonstrated efficacy against certain pathogens. By further optimizing our handling and storage practices, we could enhance the isolation of potent antimicrobial compounds from these materials; this would provide valuable alternatives in combating antibiotic resistance.