The Journal of Phytopharmacology 2024; 13(6):464-469 DOI:10.31254/phyto.2024.13607

Research Article

Comparative analysis of Albizia lebbeck and Lawsonia inermis: Phytochemical estimation and their immunomodulatory effects

Salma Osman Mohamedkhair Osman¹ , Lata Paliwal¹ , Sonu Ambwani¹ , Tanuj Kumar Ambwani²

Salma Osman Mohamedkhair Osman,Lata Paliwal,Sonu Ambwani,Tanuj Kumar Ambwani

1. Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India
2. Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India

*Author to whom correspondence should be addressed.

Received: 16th October, 2024 / Accepted: 6th December, 2024 / Published : 31st December, 2024

Abstract

Indigenous knowledge holds valuable insights into medicinal plants and their traditional uses. Incorporating products derived from medicinal plants as natural antioxidants could play a crucial role in enhancing immunity and overall health. This study investigates the immunomodulatory effects of two plant hydromethanolic extracts viz., Albizia lebbeck (ALE) and Lawsonia inermis (LIE), utilizing a chicken lymphocytes culture model. Both plants were extracted using a hydromethanolic method, revealing significant phytochemical constituents, including flavonoids and phenolics. The percent yield of LIE (6.08%) was higher than that of ALE (5.44%). Phytochemical analysis indicated that ALE contained 124 µg rutin equivalents (RE)/mg of extract for flavonoids and 3.93 µg gallic acid equivalents (GAE)/mg for phenolics, while LIE showed 104.5 µg RE/mg and 1.84 µg GAE/mg, respectively. The maximum non-cytotoxic dose (MNCD) was established at 0.4 mg/ml for ALE and 0.2 mg/ml for LIE, beyond which both the extracts exhibited dose-dependent cytotoxicity. Both extracts enhanced T and B cell proliferation, significantly. These findings suggest that ALE and LIE possess significant immunomodulatory properties, potentially offering therapeutic applications in managing immunity-related conditions. Further research is warranted to elucidate their mechanisms of action and establish optimal dosages for clinical use.