The Journal of Phytopharmacology, 2021;10(5):357-361 DOI:10.31254/phyto.2021.10513

Research Article

Phenolic and Flavonoid contents, DPPH antiradical scavenging and antidiabetic activities of the hydromethanolic extract of Nyambaka propolis

Cecile Fabiola Balingui¹ , Talla Ernest Rodrigue² , Ngassoum Martin Benoît¹ , Talla Emmanuel³ , Awalou Oumarou²

Cecile Fabiola Balingui,Talla Ernest Rodrigue,Ngassoum Martin Benoît,Talla Emmanuel,Awalou Oumarou

1. Department of Applied Chemistry, National Advanced School of AgroIndustrial Science, University of Ngaoundere, 455,Ngaoundere, Cameroon
2. Department of Biological Science, University of Ngaoundere, 455, Ngaoundere, Cameroon
3. Department of Chemistry, Faculty of Science, University of Ngaoundere, 455, Ngaoundere, Cameroon

*Author to whom correspondence should be addressed.

Received: 20th August, 2021 / Accepted: 7th September, 2021

Abstract

This study proposes an alternative treatment based on Nyambaka propolis which can reduce the oxidative stress and hyperglycaemia caused by streptozotocin. Standard protocols were used to perform phytochemical screening. In vitro experiment was assessed to quantify the total of phenolic and flavonoid contents. The antioxidant activity of the extract was evaluated by the DPPH (2,2–diphenyl-1- picrylhydrazil) antiradical scavenging assay. Diabetes mellitus was induced using single intraperitoneal injection of streptozotocin (100mg/Kg). The phytochemical screening revealed the presence of compound such as: saponins, phenolics, flavonoids, terpenoids and steroids. The total of phenolic and flavonoid contents was respectively (893.1±0.5mg G.A.E/100g of extract) and (37.5±0.2 mg Q.E/100g of extract). The hydromethanolic (95/5) of propolis extract inhibited DPPH free radicals at (78.48±0.06%) with an IC50 of (0.096 mg/ml). Concerning antidiabetic activity, the dose of 500mg/Kg of propolis extract had the highest percentage reduction in glucose levels that means (78.13%) compared to the dose of 250 mg/Kg (65%) and the dose of 20mg/Kg of gibenclamid (77.6%). The hydromethanolic extract of Nyambaka propolis can be recommended to reduce the oxidative stress and hyperglycaemia caused by streptozotocin.