The Journal of Phytopharmacology, 2020;9(5):367-370 ; DOI:10.31254/phyto.2020.9513
Screening for cytotoxic activity of Habenaria longicorniculata J graham tubers- an in vitro study
Mallya Suma V1 , BN Satish2 , Vishwanatha3
1. Associate professor, Department of PG Studies in Dravyaguna, SDM College of Ayurveda, Kuthpady, Udupi, Karnataka-574118, India
2. PhD Scholar and Professor, Sri Dharmasthala Manjunatheshwara College of Ayurveda, Kuthpady, Udupi, Karnataka, India
3. Research Officer, Sri Dharmasthala Manjunatheshwara College of Ayurveda, Kuthpady, Udupi, Karnataka, India
*Author to whom correspondence should be addressed.
Received: 31st August, 2020 / Accepted: 11th October, 2020
About: Habenaria longicorniculata J. Graham are tuberous orchid, the tubers utilized by flok healers in cancer managemnet, as a rejuvenator. A study has been planned to evaluate In-vitro cytotoxicity of tuber extract against selected cell lines. Materials and Methods: H. longicorniculata J.Graham identified, uprooted during their flowering time. Tuber extract of this plant used for its In-vitro cytotoxicity against selected cell lines of Human Breast cancer (MCF 7), Human Liver carcinoma (HepG2), and Human cervix adenocarcinoma (HeLa) cells as per standard protocol. Results: Tuber Extract exhibited a CTC50 value of >1000 on MCF 7, HepG2 and HeLa cell lines. The results from the MTT assay indicate that 72hr extract incubation with the combined extracts is toxic to the cells and the level of damage is concentration dependent.
Habenaria longicorniculata, In-vitro study, Cytotoxic activity, Human Breast cancer (MCF 7), Human Liver carcinoma (HepG2), Human cervix adenocarcinoma (HeLa).
HOW TO CITE THIS ARTICLE
Satish BN, Mallya SV, Vishwanatha. Screening for cytotoxic activity of habenaria longicorniculata J graham tubers-an in vitro study. J Phytopharmacol 2020; 9(5):367-370.
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