The Journal of Phytopharmacology, 2021;10(4):225-229 DOI:10.31254/phyto.2021.10402

Research Article

Phytochemical Screening and Thin Layer Chromatography Profiling of Various Extracts of Achyranthes aspera and Cissus quadrangularis

V Sureshkumar¹

V Sureshkumar

1. Assistant Professor and Head, Department of Veterinary Pharmacology and Toxicology, Veterinary College and Research Institute, Tirunelveli-627 358, Tamil Nadu Veterinary and Animal Sciences University, India

*Author to whom correspondence should be addressed.

Received: 26th May, 2021 / Accepted: 19th June, 2021

Abstract

Achyranthes aspera and Cissus quadrangularis are important medicinal plants possessing wide biological activity. The natural products obtained from plants provides an unique opportunity for the development of new drugs but due to their complex nature there is a need to isolate and purify the bioactive compounds from plant extracts by separation techniques. Our earlier findings revealed that methanol extracts of A. aspera and C. quadrangularis and aqueous extract of C. quadrangularis exhibited antibacterial activity. This prompted to take up further research to reveal these plants’ potential valuable phytochemicals and therefore an attempt has been made to separate the phytochemical constituents of methanol and aqueous extracts of A. aspera and C. quadrangularis by thin layer chromatography. Phytochemical screening of both plant extracts revealed more concentrated phytochemicals in methanol extracts than in aqueous extracts. The TLC profiling showed that methanol extract of A. aspera and C. quadrangularis are rich in flavonoids and phenolic compounds. Flavonoids were separated with n-butanol:ethyl acetate:water (1:2:3) solvent system at Rf value of 0.66 and was confirmed by post-development derivatization with 3% boric acid and 10% oxalic acid spray. After derivatization, the flavonoid spot could be further visualized under UV light at 254nm. Phenolic compounds were separated with methanol:water (2:1) solvent system at Rf value of 0.88 and confirmed with 10% ferric chloride spray. From the present study, suitable mobile phase for separation of flavonoids and phenolic compound fraction from methanol and aqueous extracts of both plants and TLC profiling have been developed.